(ITRs) required for AAV production. To minimize recombination, we propagate these plasmids in Stbl2 cells from Invitrogen. Also, to minimize recombination, cells should be cultured at 30 C. Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are ...
product name : AAVS1-TALEN-R catalog : 59026 more info or order : Addgene product webpage citations: 30 Reference Bury L, Fu S, Wynshaw Boris A. Neuronal lineage tracing from progenitors in human cortical organoids reveals novel mechanisms of human neuronal production, diversity, and di...
Visit our viral production page for moreinformation. Information for AAV9 (Catalog # 105558-AAV9)(Back to top) Purpose Ready-to-use AAV9 particles produced from pENN.AAV.CamKII 0.4.Cre.SV40 (#105558). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII 0.4...
Retrograde functionality is dependent on high viral titers. Addgene recommends not diluting your AAV preps prior to use. Using FLEX vectors in vivo:LoxPsites in FLEX plasmids are known to recombine during DNA amplification and viral vector production, which may result in a minority of Cre-activate...