Editing may regulate RNA abundance and fate by changing the local structure22. This may change the stability of the RNA duplex, affect structural determinants recognized by trans-acting factors and can change accessibility of factors to defined regions23,24,25. As even a single-nucleotide variant ...
ADAR1 forms a complex with Dicer to promote microRNA processing and RNA-induced gene silencing. Cell (2013) 153:575-89. doi:10.1016/j.cell.2013.03.024Ota, H. et al. ADAR1 forms a complex with Dicer to promote microRNA processing and RNA-induced gene silencing. Cell 153, 575-589 (...
Newly transcribed RNA usually dissociates from its template DNA strand immediately after transcription, but occasionally it forms a stable RNA:DNA hybrid, which consequently leaves the sense DNA in a single-stranded form. This structure, called an R-loop, often spans 100–2000 bp and causes ab...
Structure of the DLM-1-Z-DNA complex reveals a conserved family of Z-DNA-binding proteins. The first crystal structure of a protein, the Z alpha high affinity binding domain of the RNA editing enzyme ADAR1, bound to left-handed Z-DNA was recently... T Schwartz,J Behlke,K Lowenhaupt,....
& Rich, A. Structure of the DLM-1–Z-DNA complex reveals a conserved family of Z-DNA-binding proteins. Nat. Struct. Biol. 8, 761–765 (2001). Article CAS PubMed Google Scholar Herbert, A. Z-DNA and Z-RNA in human disease. Commun. Biol. 2, 7 (2019). Article PubMed PubMed ...
In double-stranded regions of RNA, the I-U base pair is unstable compared to the parental A-U base pair, leading to changes in secondary structure of RNA that can result in degradation (Wang et al., 2013). In mammals, ADAR1 edits coding RNAs (Hartner et al., 2004), microRNAs (Yang...
It also is not yet known whether naturally occurring I-containing RNAs, similar to the synthetic IU-dsRNAs, or cytosolic RNA sensor components of the RIG-like receptor–IPS signaling complex, also associate with SG-like complexes to modulate gene expression and the innate immune response....
(ADAR1)isanRNAeditingenzyme.Itcan changethestructureandfunctionofmiRNA.Inthisstudy,weinvestigatetheroleofADAR1inregulation ofmiRNA-149*inmelanoma.Western-blotanalysiswasusedtoanalyzetheexpressionofADAR1p150, ADAR1p110andGSK3aatproteinlevel.TheexpressionofADAR1p150,miR-149*andGSK3aatmRNA levelwere...
The RNA-editing enzyme adenosine deaminase acting on RNA 1 (ADAR1) limits the accumulation of endogenous immunostimulatory double-stranded RNA (dsRNA)1. In humans, reduced ADAR1 activity causes the severe inflammatory disease Aicardi–Goutières syndrome (AGS)2. In mice, complete loss of ADAR1 ...
The RNA-editing enzyme ADAR1 is essential for the suppression of innate immune activation and pathology caused by aberrant recognition of self-RNA, a role it carries out by disrupting the duplex structure of endogenous double-stranded RNA species1,2