Oligonucleotide (oligo) sequences of Illumina adapters used in AmpliSeq, Nextera, TruSeq, and TruSight library prep kits.
1. https://support.illumina.com.cn/downloads/illumina-adapter-sequences-document-1000000002694.html 下载上图文件。 2.寻找自己的建库试剂盒,查找adapter 3.查找illumina 官方文件 4.完成。
在将测序结果比对到基因组(read mapping)前,Adapter必须被移除。以前的解决方法操作复杂或相关功能不全,尤其是对于 colour-space data(后缀为.csfasta & .qual的测序文件)。 我们开发了一款简单的命令行工具:Catadapt,它支持454,illumina和SOLiD(colour space)测序数据,具有两种去除接头的算法和其它优点。 Cutadapt官网...
Diagram of Illumina protocol using single read adapter sequencesCtcttccgatc, AcactctttccctacacgacGenomic, Ligate
在Cutadapt开发过程中，利用已发表数据对454测序数据进行测试，并针对插入、删除和修剪进行功能验证。Cutadapt支持有空白的对齐，并在短RNA数据分析中应用于colour-space reads修剪。此外，Cutadapt同样适用于illumina测序结果。运行Cutadapt时，会匹配多个接头，移除匹配度最高的接头。该工具能进行多次搜索和移除一...
For genome assembler inputs, I recommend discard set 1, treat set 2 as unmated, and treat set 3 as mated. For example: $ sortPairedReads s1.fastq s2.fastq [0] Illumina_PE-1 found 0 times [1] Illumina_PE-2 found 0 times [2] Illumina_PE-1rc found 54 times [3] Illumina_PE-2...
The program Flexbar preprocesses high-throughput sequencing data efficiently. It demultiplexes barcoded runs and removes adapter sequences. Several adapter removal presets for Illumina libraries are included. Flexbar computes exact overlap alignments using SIMD and multicore parallelism. Moreover, trimming ...
Trimmomatic它包括如下功能：ILLUMINACLIP: Cut adapter and other illumina-specific sequences from the read.SLIDINGWINDOW: Perform a sliding window trimming, cutting once the average quality within the window falls below a threshold.LEADING: Cut bases off the start of a read, if below a ...
Li, H. Minimap2: pairwise alignment for nucleotide sequences. Bioinformatics 34, 3094–3100 (2018). Article CAS Google Scholar Chaisson, M. J. P. et al. Multi-platform discovery of haplotype-resolved structural variation in human genomes. Preprint at bioRxiv https://doi.org/10.1101/19314...
Cutadapt removes adapter sequences from high-throughput sequencing reads M Martin - 《Embnet Journal》 被引量: 5698发表: 2011年 Investigate and analyze the bioinformatics databases and their utilization To investigate and analysis the present situation of bioinformatics databases from the viewpoint of ...