Palmitoylation entails the incorporation of a 16-carbon palmitate moiety to cysteine residues of target proteins in a reversible manner. Here, we describe a biochemical approach, the acyl-PEGyl exchange gel shift (APEGS) assay, to investigate the palmitoylation state of any protein of interest in ...
Systematic Screening of Depalmitoylating Enzymes and Evaluation of Their Activities by the Acyl-PEGyl Exchange Gel-Shift (APEGS) Assay. 来自 Springer 喜欢 0 阅读量: 92 作者:Takashi,Kanadome,Norihiko,Yokoi,Yuko,Fukata,Masaki 摘要: Palmitoylation is a reversible posttranslational lipid modification of...