The alternative method of acyl-RAC was also used to assess the palmitoyl-proteome of rat brain material. Our initial attempt using rat brain homogenate and the published control treatment of 2 M NaCl21was unsuccessful. However, changing the control treatment to 2 M Tris.HCl, as in ABE, ...
The microscale thermophoresis binding assay revealed that the purified GST-MrACBP2 had the highest affinity for palmitoyl-CoA (Kd =70.57 nM). Further, theMracbp2gene was homologously overexpressed inM. ruberCICC41233, and a positive transformantM. ruberACBP-E was isolated. In theMonascuspigments ...
货号:60124 包装:U 品牌:KiKKoman 龟甲万 N-酰基甘露糖胺1-脱氢酶;来源:recombinant E. coli;活性:30 U/mg lyophilizate 微信号 15221999938 联系我们 上海金畔生物科技有限公司 电话:021-50837765 手机:15221999938 网址: www.jinpanbio.com Email:sales@jinpanbio.com ...
fractions. The amount of palmitoylated protein was determined using Acyl-RAC. For each fraction, palmitoylated proteins were detected after hydroxylamine treatment (+HA). Equal volumes were by analyzed SDS-PAGE and immunoblotting with anti-GFP antibodies. e, Cells were treated for 4 h with MG...
to be the main biosynthetic enzyme of 2-AG in the central nervous system. While the role of DAGL in the hydrolysis of membrane phospholipid-derivedsn-1,2-diacylglycerol species is well established, it was described that DAGL enzymes are unlikely to be involved in the degradation ofrac-1,3...
In contrast, C(4)-HSL had negligible influence on the phosphorylation status of PLC and Rac1/Cdc42 and failed to attract neutrophils and induce calcium release. The calcium inhibitor thapsigargin, which blocks ER calcium uptake, strongly prevented neutrophil migration toward 3O-C(12)-HSL and -...
ACBD3 was identified in a mammalian two-hybrid assay as a host protein that utilizes its C-terminal GOLD region to bind the Aichi virus nonstructural proteins 2B, 2C, and 3A [26]. Four hours after infection, ACBD3 was found to colocalize with 2B, 2C, and 3A in clusters in the cyt...
>98%ee, all of the following requirements should be fulfilled (Fig. 2.3.B): (1) the KR should be highly enantioselective (E value=kfast/kslow>100); (2) the interconversion of two enantiomers, i.e., the racemization (krac), should be faster than the fast-reacting enantiomer's ...
The DGAT activity assay was identical to the WS assay except that the reaction mixture contained 3.75 mm1,2-dipalmitoyl-rac-glycerol instead of 1-hexadecanol. Here triolein was used as the unlabeled TAG reference substance. Acyl-CoA specificity of the WS and DGAT reactions was assayed in a ...
The VLDL fraction (3 ml) was extracted (51), and after the addition of internal standard (4 μg, 1,2-di-O-hexadecyl-rac-glycerol) and 2 ml of acetone (with 0.01% butylated hydroxytoluene), it was heated in a culture tube with a Teflon-lined cap at 90° for 30 min. Ethyl ...