Acyl-CoA binding (ACB) proteins specifically bind both medium-chain and long-chain acyl-CoA esters. However, the role of the Acb protein in plant-pathogenic fungi has not yet been investigated. Here, we identified MoAcb1, a homolog of the Acb protein in Saccharomyces ...
1992. Molecular cloning of the gene for the yeast homolog (ACB) of diazepam binding inhibitor/endozepine/acyl-CoA-binding protein. Proc. Natl. Acad. Sci. USA 89: 1287–1291. Google Scholar Sambrook, J., Fritsch, E.F. and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual, ...
Acyl-CoA-binding protein has been isolated independently by five different groups based on its ability to (1) displace diazepam from the GABAA receptor, (2) affect cell growth, (3) induce medium-chain acyl-CoA-ester synthesis, (4) stimulate steroid hormone synthesis, and (5) affect glucose-...
Identification and functional expression of a type 2 acyl-CoA:diacylglycerol acyltransferase (DGAT2) in developing castor bean seeds which has high homolog... Seed oil from castor bean ( Ricinus communis) contains high amounts of hydroxy fatty acid rich triacylglycerols (TAGs) that can serve as ...
Also present is a sequence that encodes a homolog of E. coli plsX, a gene that plays a poorly understood role in phospholipid synthesis. The B. subtilis plsX gene weakly complemented an E. coli plsX mutant. The order of genes in the cluster is plsX fabD fabG acpP, the same order found...
Panel B: Binding of acyl-CoA to BnDGAT1(1–116)His6 demonstrated using non-denaturing gel electrophoresis and phosphorimaging. Phosphorimage obtained using radiolabeled erucoyl-CoA (lane 1) and BnDGAT1(1–116)His6 incubated with radiolabeled erucoyl-CoA (lane 2). Protein stain of BnDGAT1(...
The transcriptomes of the two cell types in the late log phase showed that the majority of the genes involved in aceticlastic methanogenesis were downregulated in the fila- ments (Supplementary Table S1), such as the CO dehydrogenase/acetyl-CoA synthase operon and the electron transfer chain F420...
MadAN malonyl-CoA decarboxylase activity was not detected (<6.9 × 10−5 pmol/min/pmol protein). ACP, acyl carrier protein; GNAT, GCN5-related N-acetyl transferase; Mad, malonyl-ACP decarboxylase. The thermal denaturation profiles of the recombinant proteins showed MadA has two distinct ...
First, we apply CATNIP to identify acetyl-CoA-binding proteins through unbiased clustering of competitive dose-response data. Next, we use this method to profile the selectivity of acyl-CoA/protein interactions, leading to the identification of specific acyl-CoA engagement signatures. Finally, we ...
J. 1992. Molecular cloning of the gene for the yeast homolog (ACB )of diazepam binding inhibitor/endozepine/acyl-CoA-binding protein. Proc. Natl. Acad. Sci. USA 89:11287–11291. Roughan, P. G., Holland, R. and Slack, C. R. 1979. On the control of long-chain fatty acid ...