Observations of activity in Afar show that dyking rather than faulting is the primary mode for accommodating extension in the elastic upper crust in mature rift systems where sufficient magma supply is available57. The behaviour of these magmatic systems is analogous to observations from spreading cent...
Exclusion ChromatographyLiquid chromatography tandem-mass spectrometry (LC-MS/MS)Muhialdin et al. (2016) Gel filtration chromatography and preparative HPLCHPLCLuz et al. (2017) Enterocin–Treatment with different enzymesBen Braiek et al. (2018) ...
Trastuzumab and pertuzumab combination has been approved for the treatment of patients with HER2-positive metastatic breast cancer. However, trastuzumab and pertuzumab combination did not show improvement in overall survival in patients with HER2-positiv
suggesting that amplification could have been caused by either transposition to different sites in the genome or tandem amplification driven by the homologous copies of ISSau134. To discriminate between these possibilities, we used ISmapper35to search for new transposon insertion sites in ...
1A, mass peptide fingerprinting using liquid chromatography-tandem mass spectrometry following trypsin digestion was performed on the major bands identified by SDS-PAGE. Tryptic digestion of the 87-kDa hLOLX2 band yielded 43 peptides matched to 77 unique spectra, which gave sequence coverage of 64...
Simultaneous measurement of cyclosporin A and tacrolimus from dried blood spots by ultra high performance liquid chromatography tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2012;883–884:102–7. Article PubMed Google Scholar Shokati T, Bodenberger N, Gadpaille H, Schn...
Methylation of proteins at arginine residues, catalysed by members of the protein arginine methyltransferase (PRMT) family, is crucial for the regulation of gene transcription and for protein function in eukaryotic organisms. Inhibition of the activity o
Proteins were identified by peptide sequencing using nanospray ion-trap tandem mass spectrometry (nESI-IT MS/MS). The nESI-IT MS/MS experiments were performed on an LC Packings (Dionex)/4000 Q Trap (Applied Biosystems) in positive ion mode. Protein identification was carried out using the PMF...
mM HEPES (pH 8.5), and labeled with TMT reagents (Thermo Scientific). The differentially labeled samples were pooled equally, desalted, and dried for the subsequent peptide fractionation. Peptide analysis by two-dimensional liquid chromatography–tandem mass spectrometry (LC/LC–MS/MS) and MS...
Liquid chromatography coupled to tandem mass spectrometry To identify acetylation site(s) on RRM2, Flag-tagged human RRM2 was overexpressed in HeLa cells. Cells were then treated with TSA and NAM for 16 h. Overnight in-gel trypsin digestion was performed on the immunoprecipated Flag-RRM2 reso...