Diana M Carlsbad, NM135 contributions 0 Guide was amazing! Mr. Banks was friendly, helpful, and very knowledgeable. Didn't see any manatee. They have already started migrating south. But the kayak trip was so peaceful and pretty. There are plenty of birds to watch. The mosquitoes and no...
In “Results” section, we assess reconstructions when the mutation ordering is inferred based on mutation VAF. In this scenario, reconstruction errors can occur when (i) cancer cell fraction (CCF) estimates are inaccurate and, (ii) there are two SNV clusters which overlap in CCF space but ha...
DNA extraction from sediment samples was performed using the PowerSoil® DNA Isolation Kit (MoBio Laboratories Inc., Carlsbad, USA) following the manufacturer’s Alternative PowerSoil Protocol for RNA and DNA from Low Biomass Soil. The DNA quality was assessed by ratios of 260 nm/280 nm and ...
DNA extraction from sediment samples was performed using the PowerSoil® DNA Isolation Kit (MoBio Laboratories Inc., Carlsbad, USA) following the manufacturer’s Alternative PowerSoil Protocol for RNA and DNA from Low Biomass Soil. The DNA quality was assessed by ratios of 260 nm/280 nm and ...
The plasmid (β- catenin) was transfected using Lipofectamine Plus (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's guidelines. Centella asiatica extract (CAE), as a positive control, was obtained from Korea Chong Kun Dang Pharma. Co. Ltd., in Korea. Plant material. S...
Sytox Green was from Molecular Probes (Invitrogen, Carlsbad, CA, USA). Download: Download high-res image (47KB) Download: Download full-size image Scheme 1. Amino acid sequence of Esc(1-21) and Esc(1-21)-1c. The d-amino acids at position 14 and 17 are shown in green and in ...
The DPPH radical scavenging activities of non-treated and enzyme-treated WSGL were determined by mixing 0.1 mM DPPH with samples or Trolox standard (0–250 µM) in a 96-well plate. The reaction mixture was incubated at 28 °C for 30 min. The plate was read at 517 nm by the SpectraM...
Thus exploration and production of mineral resources should be compatible with, and complementary to, deep geologic waste disposal.N. T. RempeWestinghouse Electric Corp., Carlsbad, NM (United States)
Fetal calf serum and modified McCoy's 5A medium were purchased from Gibco (Carlsbad, CA, USA). The One-step PCR kit was purchased from TaKaRa Biotechnology (Dalian, China). Oligonucleotide primers were customly synthesized by Sangon Biological Engineering Technology (Shanghai, China). All other...
and dissolved in dimethyl sulfoxide for further study. Norfloxacin was bought from Aladdin (Shanghai, China). The Cytotoxicity Detection Kit (Roche) was purchased from Sigma Aldrich Inc. (St. Louis MO, USA). The Live/Dead BacLight Bacterial Viability Kit was bought from Invitrogen (Carlsbad, ...