An officer in theU.S. Air Forcewas crowned Miss America on Sunday night. Second Lt. Madison Marsh is the firstactive-duty service memberto win the annual competition, accordingto the Air Force. Marsh represented Colorado, the home of theU.S. Air Force Academy, in...
4 cerebella was immediately homogenized in ice-cold homogenization buffer (10 mM HEPES [pH 7.4], 150 mM KCl, 5 mM MgCl2, 0.5 mM dithiothreitol (DTT), 100 μg/ml cycloheximide, Complete-EDTA-free protease inhibitors (Roche) and RNasin RNase inhibitors (Promega, Madison, WI) using a Teflon...
nucleofected with pNL4-3, AG49CMVGag-RTEm26CTE, AG49CMVGagW16A-RTEm26CTE, AG49CMVGagW36A-RTEm26CTE, AG49CMVp17-RTEm26CTE, and AG49CMVp17G2A-RTEm26CTE using the Amaxa Nucleofector II device (Lonza, Amboise, France) and the Ingenio Electroporation Universal kit (Mirus, Madison, USA...
pIEx-1 Ek/LIC vector, Escherichia coli strain NovaBlue, BL21(DE3), and Insect Gene Juice were obtained from Novagen (Madison, WI). Total mRNA from adult Dme, Taq HiFi polymerase, deoxynucleoside triphosphates, Sf9 cells, and Sf-900 II SFM medium were purchased from Invitrogen Corp. (...
In Proceedings of the Fifth National Conference of IBPSA-USA, Madison, WI, USA, 1–3 August 2012. [Google Scholar] Kummert, M.; André, P.; Nicolas, J. Optimal heating control in a passive solar commercial building. Sol. Energy 2001, 69, 103–116. [Google Scholar] [CrossRef] Olde...
Cells were plated in 96 wells treated with different concentrations of pAXL×CD3ε, and cell viability was evaluated by Cell Titer-Glo Luminescent Assay (CTG; Promega, Madison, WI, USA), as previously reported [41]. 2.8. Western Blot Whole-cell protein extracts were obtained using NP40 lysis...