We scanned the femur using in vitro X-ray microtomography (Skyscan 1272, Bruker micro CT) at a pixel size of 9 µm, and analyzed the results according to the manufacturer’s instructions. Region-of-interest (ROI) was defined from 10 to 110 image slices, where the growth plate slice...
function of the mammalian central pacemaker, the suprachiasmatic nucleus (SCN), show similarities with the fly pacemaker neurons, such as the importance of peptidergic signaling11and cAMP12. These similarities in molecular and neuronal mechanisms suggest that studies in flies can shed light on the mec...
Analysis of pituitary cell lineage commitment factors at E15.5 revealed that the number of TPit+ve cells was increased in theProp1:Cre;BrafV600E/+andCAG:Cre; BrafQ241R/+embryos compared to Wt (Fig.7a, d, g, j). The Pit1 lineage transcription factor appeared severely reduced in theProp...
Vaudry D, Falluel-Morel A, Bourgault S, Basille M, Burel D, Wurtz O, Fournier A, Chow BK, Hashimoto H, Galas L, Vaudry H: Pituitary adenylate cyclase-activating polypeptide and its receptors: 20 years after thediscovery. Pharmacol Rev. 2009, 61 (3): 283-357. 10.1124/pr.109.001370....
DESeq2 R package was employed for differential expression analysis between groups, adjusting p-values with Benjamini and Hochberg's method to manage the false discovery rate. Genes were deemed differentially expressed with p<0.05 as per DESeq2. An adjusted p-value of 0.05 was the criteria for ...
Phenotypic screening models for rapid diagnosis of genetic variants and discovery of personalized therapeutics 2023, Molecular Aspects of Medicine Citation Excerpt : Nevertheless, in silico approaches are rapidly evolving. For instance, the Genome-to-Protein Informatics Program (G2PIP) at the Mayo Clini...
(cut-off by false discovery rateq-value < 0.05) (GSE171115) (Additional file1: Fig. S4a, b). This analysis identified gene sets co-regulated by hypoxia and METTL4, which contain enriched hypoxia-related and the epithelial-mesenchymal transition programs among the major pathways (Additional ...
GTPase activating proteins: structural and functional insights 18 years after discovery Cell. Mol. Life Sci, 62 (2005), pp. 3014-3038 CrossrefView in ScopusGoogle Scholar 13 E. Ligeti, S. Welti, K. Scheffzek Inhibition and termination of physiological responses by GTPase activating proteins Phys...
Solid Tumour Target Discovery Laboratory, Newcastle Cancer Centre, Northern Institute for Cancer Research, Medical School, Newcastle University, Newcastle upon Tyne, UK Urszula L. McClurg Newcastle Cancer Centre at the Northern Institute for Cancer Research, Newcastle University, Paul O’Gorman Building,...
Lamtor1 antibody. LC–MS/MS of immunoprecipitates was performed on Q-Exactive and UltiMate 3000 Nano LC systems (Thermo Fisher Scientific, Yokohama, Japan). Data were analyzed using the Scaffold software (version 4.4.3) to obtain a list of protein identifications with a false discovery rate of...