When the composition is not known, buffer values may be experimentally determined from the slope of the titration curve. At any pH, the total buffer value of a solution is the sum of the buffer values of the individual constituents of that solution. The use of these principles offers an ...
aAfter preincubation, 50 μl of 5mMpNPG solution in 0.1 Mphosphate buffer (pH 6.9) was added to each well at timed intervals. 在预孵化以后, 50 5mMpNPG解答μl在0.1 Mphosphate缓冲 (酸碱度6.9) 很好增加了到其中每一在计时的间隔时间。[translate] ...
A.MildsteelCitrateBipyridineOscillatingreactionIronphosphateTo study the effect of strong iron-ligands on steel corrosion, mild steel electrodes were immersed in solutions containing 20 mM phosphate buffer (pH=7.2) and between 0.01 mM and 1 M of either the iron(II)-chelators 2,2′-bipyridine or...
data examines the evolution of the contours of membranes patches exposed to AMPs in solution. Never have the dynamics of the activity of AMPs been imaged at molecular resolution, for example oligomers could never be visualised due to their thermal diffusive motion. AFM and EC-STM molecular imaging...
action of rennets on the caseins. I. Rennin action on beta-casein -B in solution The primary proteolysis of caseins by chymosin in Gouda cheese was assessed by identifying the peptides present in the watersoluble fraction of a starter-f... LK Creamer,OE Mills - 《Journal of Dairy Rese...
were stained with 10 μM DCFDA in Hank's balanced salt solution for 30 min in the darkness, and then the fluorescence was measured by a microplate reader (Beckman Coulter DTX 880 Multimode Detector, Brea, CA, USA) at an excitation wavelength of 485 nm and an emission wavelength of 525 ...
MOLECULAR MECHANISM OF α1(I)-OI/EDS: UNFOLDING OF AN N-ANCHOR DOMAIN AT THE N-TERMINAL END OF THE TYPE I COLLAGEN TRIPLE HELIX sodium carbonate/0.5 M NaCl (pH 9.3). Pepsin- treated collagen was transferred into the same buffer by mixing a 2 mM HCl solution of the protein 1:1 with...
Interactions between maltodextrin (DE = 10) and an anionic surfactant (sodium dodecyl sulfate, SDS) were studied in a buffer solution (pH 7.0, 10 mM NaCl, ... A Wangsakan,P Chinachoti,DJ Mcclements - 《J Agric Food Chem》 被引量: 50发表: 2001年 Characterization of Cyclodextrin Glycosyltra...
For flow cytometry, 40 μl blood sample from each mouse was pipetted into a centrifuge tube containing 10 μl of 3.2% sodium citrate solution (1: 4) and further diluted (1: 20) in 1 ml modified Tyrode's buffer. The diluted blood sample then was centrifuged at 900×g for 5 min at...
a result of this interaction, the N-terminus (His-7 to His-10) points deep into the active site and extends towards the catalytic zinc. This second interaction is unlikely to contribute to the inhibition of LC/A1, because there is no evidence of tetrameric complexes in solution (...