To further confirm this result, we chose these grown yeast colonies from SC/–uracil plates and diluted them into a new SC/–uracil plate to compare their growth activity. As shown in Figure 4, we finally identified M20, containing a short sequence of 174 nt located in between V1 and C3...
3.8. SYTOX Green Uptake Assay Pre-grown E. coli cells were washed and suspended to 2 × 107 cells/ ml in SP buffer. The cells pre-treated with 1 µM SYTOX green for 15 min in the dark were incubated with the peptides in appropriate concentrations; the bacteria were analyzed by flow...