Category Polyclonal antibody(pAb); Primary antibody Isotype IgG Purification Peptide affinity purified About the Immunogen Immunogen This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to near C-terminal residues of human AFP. Gene ID 174 SwissProt P02771...
Coverslip was rinsed four times with 1x PBT and incubated in 30 µL of 1:100 diluted secondary antibody (Goat anti-Mouse IgG Alexa Fluor plus 488, Ref A32723, Thermo Fischer Scientific, Waltham MA) for 1 h. Coverslip was rinsed with 1x PBT and mounted on a slide with 1.5 ...
2a)28,29. To particularise the organisation of the actin cytoskeleton in FADS fibroblasts, we first visualised actin SFs by phalloidin and contractile actin-myosin bundles using an antibody to non-muscle myosin II A (NMIIA). Their respective distribution was analysed automated using an ImageJ ...
The blocking solution was removed by aspiration, and the primary antibody (diluted in blocking buffer) was applied for 1 h. After three PBS washes, the primary antibody was detected by indirect immunoperoxidase staining using a Vectastain ABC Elite mouse IgG kit (Vector) as directed by the ...
Then 0.5 µg rabbit IgG, 2 µg CXCR5 antibody, 2 µg actin antibody, or 2 µg magnetic beads were incubated with 40 µL protein A/G for 2 h at 4°C and washed four times with PBS (0.05% PBST), using 0.05% Triton X-100. The pretreated protein samples were then mixed ...
Interestingly, blocking BDNF with a BDNF antibody significantly decreased AnkG staining and the polarity index (Figure 7B). These results suggest that BDNF-TrkB signaling is involved in the regulation of ankG localization. Next, we examined whether local activation of Opto-cytTrkB regulates the ...
Source: Mouse, Monoclonal/IgG1, Kappa Dilution: IF 1:50-200 WB 1:5000-20000 Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. Concentration: 1 mg/ml Storage Stability: -15°C to -25°C/1 year(Do not lower than -25...
Sections were washed with PBS, then incubated in secondary antibody for 5 h at room temperature. After further extensive washing, sections were mounted onto slides using Gene Frame gaskets (Advanced Biotechnologies) and were sealed with coverslips. The samples were examined on a Leica DM-IRBE ...
Primary Antibody (green line): Rabbit Anti-beta-Actin (Loading Control) antibody (bs-0061R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA...
34. Our finding that stereocilia lengths in thejordanmouse were only marginally longer than those in theshaker-2led us to hypothesize that MYO15A trafficking was defective injordanhair cells. We used the previously validated pan-MYO15A antibody PB48 (Fig.1A), that binds an epitope common to ...