Strikingly, ectopic expression of BrmDN and Gem together using en-GAL4 caused a dramatic developmental defect leading to lethality during the first instar larval stage (data not shown). This phenotype was much stronger than expected if Gem and BrmDN effects were just additive, indicating that ...
We thus generated hpo mutant clones throughout wing imaginal discs and knocked down msn in the posterior compartment using the hh-Gal4 driver. Yki activity was assayed by examining the expression of the expanded-lacZ (ex-Z) reporter39 and Cubitus interruptus expression was used to identify the...
pan-muscular expression ofapusingMef2-Gal4driver induces both dCryAB (compareG, G′, withI, I′) andGelexpression (H, H′, J, J′) in all muscles. Arrowheads point to LT muscles and arrows point to ectopic expression in dorsal (I) and ventral (J) muscles...
The results show there is no increase in rhodopsin production over the basal levels when pninaE-GAL4 is present to elicit NinaB BCO expression in the R1–R6 photoreceptor cells. Two other retinal-specific GAL4 driver strains confirmed and extended this result. The first of these is the ...
Live imaging. The Lms-Gal4; UAS-lifeActGFP (Lms>LAGFP) double transgenic line was generated and used for time lapse imaging of LT muscle formation in the gel mutant context. Image acquisition was performed on manually aligned living embryos at 21 °C using an inverted Leica SP8 ...
"integrity": "sha512-xAfGg1/NTLBBKlHFmnd7PlmUW9KhVQIUuSrYem9xzFUZy13ScvtyGGejaae9iAVRiRq9+Cx7DPFaAAhCpyxyPw==", "requires": { "lodash": "4.17.5" } }, "async-each": { "version": "1.0.1", "resolved": "https://registry.npmjs.org/async-each/-/async-each-1.0.1.tgz", ...
mediated germ-line transformation [33,34]. To expresstlkin the germ line, females of a selectedUASP-tlkline were crossed withGAL4-GCN4males [35]. The resulting females carrying both transgenes were crossed withUAST-tlkmales for the collection of embryos, which are described here asGCN4>tlk...
(f) Enhanced margin loss in N/+; UAS–sca/+; dpp–Gal4/+ wing. (g) Targeted expression of N had no effect on margin structures but led to extra vein differentiation in the anterior compartment (arrow). (h) When N and sca were coexpressed, the effect of sca on the wing margin ...
The genotype of the animal shown inFig. 8wasy w hsFlp/Y; ex697FRT42D ubi-GFP/FRT42D, hpo42,43,44,45,46,47; hh-Gal4/msn-RNAi. The msn-RNAi line was the verified TRiP line BSC2879146. Wandering third instar larvae of this genotype were dissected in cold 1 × phosphate-buffered...
Coinfiltration of HEN4p::HEN4-EYFP together with 35S::GAL4-ECFP did not result in any YFP signal (Figures 5D and 5J), suggesting that HUA1 was responsible for the accumulation or localization of HEN4-EYFP. Download: Download high-res image (159KB) Download: Download full-size image ...