We have previously identified a novel type of ACT domain-containing protein family, the ACT domain repeat (ACR) protein family, in itArabidopsis/it. Members of the ACR family, ACR1 to ACR8, contain four copies of the ACT domain that extend throughout the entire polypeptide. Here, we ...
Purified PXL1LRR protein (with buffer containing 10 mM Bis-Tris pH 6.0, 100 mM NaCl) was incubated with synthesized peptide mixture (0.1 mg for each; Supplementary Table 1) on ice for 1 h. Then, each mixture was subjected to gel filtration (Superdex 200, GE Healthcare) for ...
Briefly, agarose plugs containing whole-cell DNA of strain WCHEC13-8 were treated with 8 U of S1 nuclease (Fermentas, Thermo Scientific; Waltham, MA, US) and the reaction was stopped by adding 0.5 M EDTA (pH 8). PFGE was conducted with a 1% SeaKem Gold agarose gel (Lonza, Basal, ...
(2013). A zinc-binding citrus protein metallothionein can act as a plant defense factor by controlling host-selective ACR-toxin production. Plant Mol Biol 81, 1-11.Nishimura S,Tatano S,Miyamoto Y,Ohtani K,Fukumoto T,Gomi K,Tada Y,Ichimura K,Akimitsu K. 2013. A zinc-binding citrus ...
Keywords: Taiwanese tea; degree of fermentation; catechins; gallic acid; antioxidative activity; RAW264.7; protein kinase C; molecular docking 1. Introduction One of the most oft-consumed drinks in the world, especially in Asian countries [1], is tea [2,3]. Various beneficial health effects ...