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Then, sections were stained with ACSL4 (1:250, Abcam, ab155282) antibody or Cytokeratin 18 (1:200, Proteintech, 66187-1-Ig) antibody followed by staining of secondary Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Thermo Fisher, A11008), or Goat anti-...
ACSL4 polyclonal antibody 供应商 Abnova 产品货号 PAB6260 产品报价 ¥询价/100ug 产品说明书 点击查看 购买方式 在线支付宝及网银支付、支票、现金、电汇,或拨打全国免费服务电话4006800868与我们联系。 产品新闻 背景资料 提供的ACSL4 多克隆抗体为山羊来源的多抗。ACSL4 又称ACS4|FACL4|LACS4|MRX63|MRX68,其...
Afterward, membranes were blocked with 5% nonfat milk for 1 h and treated with the specific primary antibodies against Acsl4 (1:1000, abcam), Sp1 (1:200, abcam) and GAPDH (1:2000, abcam) at 4 ℃ overnight. Following conjugation to HRP-labeled secondary antibody, the bands were ...
5.抗体:Anti-ACSL4 antibody(公司:abcam货号:1002422-15);anti-beta-tubulin mouse抗体(公司:北京康为世纪生物科技有限公司 货号:01270/07223)。 本发明的实施例是为了示例和描述起见而给出的,尽管上面已经示出和描述了本发明的实施例,可以理解的是,上述实施例是示例性的,不能理解为对本发明的限制,本领域的普通技...
Then cells were incubated with c-Myc (GTX103436) (GeneTex, 1:100) primary antibody at 4 °C overnight. Subsequently, the slides were washed with PBS and Xenograft studies in nude mice Four-week-old male BALB/c nude mice were purchased from Shanghai Experimental Animal Center of Chinese ...
C ChIP‒qPCR analysis using an anti-C/EBPα antibody and the primer pairs shown in (A). The bar graphs represent the mean ± SD. *P < 0.05, ****P < 0.0001 compared with the respective experimental control; ###P < 0.0001 compared with cells under the same experimen...
All blots were incubated with HRP-conjugated secondary antibody (A0216, Beyotime, Shanghai, China) for 1 h and next visualized using Odyssey Infrared Imaging Scanner (LI-COR Biosciences). GAPDH was used as the loading control. The intensities of bands were calculated using Image-J software (...
In addition, xenograft tissue samples collected from nude mice were stained with an anti-ACSL4 antibody (#ab155282, Abcam) and anti-Ki67 antibody (#ab92742, Abcam). Determination of reactive oxygen species and lipid peroxidation levels Cellular levels of reactive oxygen species (ROS) were ...
Then, secondary antibody incubation and DAB colouration were performed using the Dako REAL EnVision Detection System (K5007, Copenhagen, Denmark). The primary antibodies used for IHC targeted FTO (1:400, Santa Cruz, USA), ACSL4 (1:500, Abcam, Cambridge, MA), and TFRC (1:1000, Abcam). ...