Phos-tag™ SDS-PAGE 操作简单,只需在常规 SDS-PAGE 胶中加入 Phos-tag™ Acrylamide 和 Mn2+或者 Zn2+即可进行实验。在电泳过程中,磷酸化蛋白的磷酸基团与 Phos-tag™ 中的二价金属离子相结合,降低其迁移速度,从而可区分磷酸化与非磷酸化蛋白。 ◆原理 ◆优点、特色 ●采用 Phos-tag™ SDS-PAGE 可...
看到丙烯酰胺的时候觉得非常熟悉，刚才在实验室反应过来原来是配SDS-PAGE蛋白胶的成分之一，这可是每个生物...
Acrylamide, stearyl methacrylate, ammonium persulfate (APS), sodium dodecyl sulfate (SDS), N,N'-bis (acryloyl) cystamine, N,N',N,N'-tetramethylethylenediamine (TEMED), and NaCl were commercially available and used as received. Structure and Properties of Hydrophobic Aggregation Hydrogel with Chemica...
英文: A novel method is reported for mass separation of proteins, based on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). 中文: 报导了一种基于十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)的新型蛋白质质量分离方法。 更详细... 英文: A COMPARISON OF SRS VIRUS PROTEINS & MOLO...
附表2. 配制SDS-PAGE分离胶 附表3. 配制5%SDS-PAGE浓缩胶 Product content component 500ml 30% Acr-Bis (29:1) 2×250ml Products 30% Acrylamide:Bis Solution (29:1) is an aqueous solution of 30% acrylamide, N,N′-methylenebisacrylamide (29:1), referred to as 30% Acr-Bis (29:1). This...
The migration rate of the proteins during SDS-PAGE is determined by the pore size of the gel matrix and charge, size, and shape of the protein. In this unit, the protocol covers the casting of gels, preparation of the protein samples, staining and drying of the gels, and calculation of...
Most acrylamide is used to synthesize polyacrylamides, which find many uses as water-soluble thickeners. These include use in wastewater treatment, gel electrophoresis (SDS-PAGE), papermaking, ore processing, and the manufacture of permanent press fabrics. Some acrylamide is used in the manufacture...
N-(2-Hydroxyethyl)acrylamide, HEAA CAS 7646-67-5 SDS including Hazard identification, Composition/information on ingredients, First-aid measures, Fire-fighting measures, Accidental release measures, Handling and storage, Exposure controls/personal protec
SDS-PAGE进行描述与PROTEAN II电泳装置(BIO-RAD)的Laemmli(1970),使用4%的浓缩胶和12%的丙烯酰胺凝胶。 翻译结果2复制译文编辑译文朗读译文返回顶部 SDS 页根据说明被 Laemmli 实行 (1970 年 ) 利用一个 Protean II 电脉单位 ( 生物拉德 ) 使用 4% 成堆凝胶体和 12% 运行 acrylamide 凝胶体。 翻译结果3复...
•Industriallyproducedvinylcompound,commonlypolymerized IndustrialUses-Flocculantforwastewatertreatment-Cosmetics,Paper&Textiles-Soilconditioner-SDS-Pagegel •1994deemeda“probablecarcinogen”•InternationalAgencyforCancerResearchviewedacrylamideonlyasasyntheticchemical •Exposurethoughttobeairborneandlimitedtotheworkplace...