Kidney cancerACKR3COPS8Mutation screeningMassively parallel sequencingHi-PlexAn apparently balanced t(2;3)(q37.3;q13.2) translocation that appears to segregate with renal cell carcinoma (RCC) has indicated potential areas to search for the elusive genetic basis of clear cell RCC. We applied Hi-...
For further subcloning of synthesized sequences, we made use of the existing pcDNA3.1(+)TM-HA-Ackr3 construct (Hoffmann et al., 2012) that carries a silent mutation generating a XhoI site (222 bp 5′ to the STOP codon of the wild-type Ackr3 sequence). This site was used for cloning...
variants displaying a leucine or a phenylalanine retained the parental activity, with Y1F mutation, mimicking the nociceptin peptide N terminus, resulting in a tenfold improvement in potency (Figs.3a, c). However, similarly to classical receptors, the phenylalanine at position 4 of the ...
Replacement of the X residue with the most frequent amino acid at this position (P10Q) had an intermediate effect between WT and P10del in each assay, with ACKR3 having a higher tolerance for this mutation. This work shows that the X residue helps to position the CXCL12 N terminus for ...
It is also noted that β-arrestin2 has been shown to be more flexible than β-arrestin1 [65,66], which may explain why mutation of some of the putative phosphorylation sites have a more pronounced effect on β-arrestin1 recruitment. In summary, we provide evidence of β-arrestin1/2 and...