By incorporating acids into the film coating syrup during processing and maintaining a pH range of 4.5 to about 6.0, the aspartame will not denature in the final film composition, despite the high processing temperatures to which the film is exposed...
Most amino acids have the basic formula RCHNH2COOH and have properties of both bases and acids, because each amino acid has an amino group (NH2) and a carboxyl group (COOH). In the simplest amino acid, glycine, R is represented by a hydrogen atom (CH2NH2COOH). In others, R may be ...
a machine-learning algorithm. The validation accuracy reaches 99.1%, with 30.9% signal recovery. The feasibility of ultrasensitive quantification of amino acids was also demonstrated at the nanomolar range. Furthermore, the capability of this system for real-time analyses of two representative post-...
The dextrorotatory-isoforms of amino acids (D-AAs) are essential components of peptidoglycan within the bacterial cell wall, providing strength and structural integrity in a diverse range of species. Among many tasks, D-AAs regulate cell morphology, spore germination, and bacterial survival, evasion...
This is not desirable because the two enantiomers of phthalidyl esters likely have different pharmacological effects. Here we address the synthetic challenges in enantioselective modification of carboxylic acids via asymmetric acetalizations. The key reaction step involves asymmetric addition of a ...
By using human-to-mouse microbiota transplantation and ex vivo analysis of colonic mucus function, we here show as a proof-of-concept that individuals who increase their daily dietary fiber intake can improve the capacity of their gut microbiota to prevent diet-mediated mucus defects. Mucus growth...
We proved this hypothesis first in rats, where we found a clear shift from AA- to EPA- and DHA-derived epoxy- and ω-hydroxy-metabolites in all major organs and tissues upon dietary EPA/DHA supplementation (22). We have now expanded our studies to humans and analyzed the effect of EPA/...
The sequence-specific formation of triplex DNA structures comprised of double-stranded DNA (dsDNA) and an oligonucleotide, known as a triplex-forming oligonucleotide (TFO), can be widely used in the molecular biology and biochemistry fields. However, current methods have not reached a practical level...
Long-chain free fatty acids (FFAs) are a type of backbone molecule that can react with alcohol to produce biodiesels. Various microorganisms have become potent producers of FFAs. Efforts have focused on increasing metabolic flux to the synthesis of either neutral fat or fatty acyl intermediates at...
Efforts to sequence single protein molecules in nanopores1,2,3,4,5have been hampered by the lack of techniques with sufficient sensitivity to discern the subtle molecular differences among all twenty amino acids. Here we report ionic current detection of all twenty proteinogenic amino acids in an...