When the OD600 reached ~1.0, the refreshed seed was transferred to a 25 mL medium in a 300 mL flask until an OD600 of 0.05 was obtained. Genes under the Ptac promoter were expressed by the addition of 0.1 mM IPTG when the OD600 reached 1. At this time, different amounts of cerule...
The acid value of the product is determined according to the method GB 5009.229-2016: Step 1, take 50 mL of ethanol solution in an Erlenmeyer flask, add 2 drops of phenolph- thalein indicator, titrate to a slight reddish color with potassium hydroxide standard solution, and note the scale...
2.6. Stirred Tank Reactor The yield of KAD synthesis in the 0.5-L stirred tank reactor (STR) (68.24 to 70.22%) when equipped with that of the two impeller design (RT and PBT) was comparable to the shake flask system (64.47%) when rotating at the same agitation speed (180 rpm) and ...
The reaction was carried out in a 25 mL round bottom flask connected to a water-cooled condenser. The reaction mixture was continuously stirred using a magnetic stirrer and refluxed at 105 ◦C. The products were analysed by GC–MS on a GCMS-QP5050A Shimadzu mass spectrometer with ionisation...
The predetermined diol (EG, PPD, or BDO), CBDO, DMFD (0.50 mol) and catalyst (Zinc acetate, 0.2 mol % based on DMFD) were added into the flask. Then the reaction was conducted at 180 ◦C for 4 h under the protection of nitrogen. After almost all the theoretically produced ...
A 10-mL flask was charged with 5 (162 mg, 0.5 mmol, 1 equiv.), Et3N (151.5 mg, 4.5 mmol, 3 equiv.), HOBT (33.7 mg, 0.25 mmol, 0.5 equiv.), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDCI) (143.2 mg, 0.75 mmol, 1.5 equiv.) and tert-butyl (5-aminopentyl)carbamate (101 ...
In cases of chemical hydrolysis, 400 g of the agricultural residue was continuously mixed with 1.2 L of 3 M H2SO4 in a 2-L triple-necked round-bottom flask with a reflux condenser for 24 h at 110 ◦C. After cooling down to room temperature, the pH value of the hydrolysate was ...
All pretreatments were conducted in a round bottom flask with a condenser. The mass ratio of the pretreatment liquor to the bamboo particles (on an o.d. basis) for all pretreatments was fitted as 15:1 (g:g). After pretreatment, the whole pretreatment slurry was separated by vacuum ...
The leachate recycling experiments were conducted in a conical flask or a sealed spherical flask by adding an appropriate amount of dry BR in 1 and 2 M H2SO4, resulting in an S/L of 10%. pH value was adjusted close to 0 with an appropriate amount of concentrated H2SO4. After 60 min...
2.3. Catalytic Measurements The esterification reaction was carried out as follows: Valeric Acid, VA (1.7 mL, 0.016 mol) and pentanol, V-OH (15 mL, 0.14 mol) were added to a 25 mL round bottom flask and stirred. The reaction mixture was heated to 120 ◦C under reflux and stirring. ...