When the OD600 reached ~1.0, the refreshed seed was transferred to a 25 mL medium in a 300 mL flask until an OD600 of 0.05 was obtained. Genes under the Ptac promoter were expressed by the addition of 0.1 mM IPTG when the OD600 reached 1. At this time, different amounts of cerule...
The reaction was carried out in a 25 mL round bottom flask connected to a water-cooled condenser. The reaction mixture was continuously stirred using a magnetic stirrer and refluxed at 105 ◦C. The products were analysed by GC–MS on a GCMS-QP5050A Shimadzu mass spectrometer with ionisation...
In cases of chemical hydrolysis, 400 g of the agricultural residue was continuously mixed with 1.2 L of 3 M H2SO4 in a 2-L triple-necked round-bottom flask with a reflux condenser for 24 h at 110 ◦C. After cooling down to room temperature, the pH value of the hydrolysate was ...
The bacteria were grown in LB medium with 100 µg/mL ampicillin overnight at 37 ◦C and continuous shaking at 150 rpm; a 2 L flask with 800 mL of LB media supplemented with ampicillin was then inoculated with 10% of overnight culture. The cultures were incubated at 37 ◦C with ...
5 mL of a 6 g/L KPS solution was added to the reaction mixture of water: DMSO (1:1,v/v), with a total volume of 50 mL. Reactions were performed at 80 °C for 3 h. Meanwhile, the flask was equipped with a condenser, and the reaction mixture was purged with nitrogen throughout...
2.6. Stirred Tank Reactor The yield of KAD synthesis in the 0.5-L stirred tank reactor (STR) (68.24 to 70.22%) when equipped with that of the two impeller design (RT and PBT) was comparable to the shake flask system (64.47%) when rotating at the same agitation speed (180 rpm) and ...
The predetermined diol (EG, PPD, or BDO), CBDO, DMFD (0.50 mol) and catalyst (Zinc acetate, 0.2 mol % based on DMFD) were added into the flask. Then the reaction was conducted at 180 ◦C for 4 h under the protection of nitrogen. After almost all the theoretically produced ...
A 10-mL flask was charged with 5 (162 mg, 0.5 mmol, 1 equiv.), Et3N (151.5 mg, 4.5 mmol, 3 equiv.), HOBT (33.7 mg, 0.25 mmol, 0.5 equiv.), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDCI) (143.2 mg, 0.75 mmol, 1.5 equiv.) and tert-butyl (5-aminopentyl)carbamate (101 ...
The diastereomeric salts were weighed and added in the barrel-type test tube with a thermometer, then the tube was set in a jacket glass flask with a temperature control. A certain amount of absolute ethanol solvent was added dropwise into the tube, and the mixture was stirred at a constant...