There are two types of acid fast staining:hot method and cold method. Ziehl-Neelsen is a hot method of acid fast staining. The components of Ziehl-Neelsen stain include primary stain (strong/concentrated carbol fuchsin), decolourizer (20% H2SO4) and counterstain (Loeffler's methylene blue)....
1.0 Principle/Indications • Mycobacterium species, particularly mycobacterium tuberculosis, are important bacteria to identify due to their ability to cause tuberculosis. Carbol Fuchsin is first applied in the acid-fast method, which stains every cell. After destaining with acid alcohol, only nonacid...
Another principle for tailor-made sialidase inhibitors is the design of low-molecular-mass compounds that mimic the binding function of a macromolecular antibody. Such studies are based on the crystal structure of an influenza virus N9-sialidase (antigen)-NC41 (antibody) complex, showing the direct...
the expression of COL2, ACAN proteins and partially relieve the injury of knee cartilage tissues from OA patient without the stimulation of IL-1β in vitro, compared with the 0 nM 10-HDA group, according to the results of toluidine blue and safranin O/fast green staining (Supplementary ...
After macroautoradiography, hematoxylin and eosin staining was performed on the same section. The heart of EMA rats was then removed and radioactivity and tissue weight were measured as described above. Dynamic PET imaging in healthy mice C57BL/6J mice (males, 9 weeks old) did not fast prior ...
(f) Hematoxylin-eosin staining of EDKO and control hearts (E18.5). Scale bar = 500 µm. (g) Heart weight/body weight ratio (%HWBW) (control = 17, EDKO = 15), body weight (g) (control = 48, EDKO = 33), and heart weight (g) (control = 17, ...
The porosity of hMSC/AL/HA-bioink and AL/HA-ink was calculated according to the Archimedes’ principle [46]. Analysis of percentage of porosity was performed by immersing the freeze-dried bioinks (0.06 ± 0.01 g) in 200 mL PBS pH 7.4 at 25 ± 0.5 °C. The submerged mass ...
The SDS-polyacrylamide gel electrophoresis of proteins was performed according to Laemmli [45] by using 8–20% gradient gels and staining with Roti®Blue quick (Carl Roth). As a protein marker, FastGene® BlueEasy Protein Marker (Nippon Genetics) was used. Native molecular weight was ...
Gomori (1941) originally devised the techniques, based on the same principle as alkaline phosphatase. As in acid pH, calcium salts are soluble in water, lead nitrate is used here instead of calcium salts and finally visualisation is brought about through the deposition of brown lead sulphide ...
44 More convenient and more effective purification can then be achieved by the same partition principle with a column of Sephadex LH-20 with similar solvent systems. Newly synthesized 16 was purified successfully with a solvent system of chloroform/methanol/2-propanol/water/triethylamine = 40:40:5...