Guarisco JA, Donnell JCO, Skovira JW, McDough JH, Shih TM. In vivo oxime administration does not influence Ellman acetylcholin- esterase assay results. Toxicol Mech Meth. 2009; 19:379-85.Guarisco JA,O'donnell JC,Skovira JW,McDonough JH Shin TM. In vivo oxime administration does not ...
For each stimulation protocol, respective control cells were observed simultaneously. The software of the live cell analyzer determined confluence values. Ellman assay This assay is based on a previously described method68, 69 with some alterations. 5,5-Dithiobis-2-nitrobenzoic acid (final ...
For cell viability assay, cells were infected with Ad.vector, Ad.AChE, ZD55 or ZD55-AChE at different MOIs for 96 hours and then subjected to cell viability detection. AChE determination Measurement of AChE activity was performed according to a modified Ellman’s assay [13]. Briefly, 1×106...
AChE activity was determined by the method of Ellman and colleagues.29 Aβ Fibril Formation Aβ fibrils were formed in a turbidity assay as previously described.23, 24 Briefly, Aβ peptide stock solution was prepared by dissolving freeze-dried aliquots of Aβ1-40 in dimethyl sulfoxide (DMSO)...
Plasma cholinesterase activities were measured by spectrophotometry assessing the rate of hydrolysis of acetylthiocholine (measured by the Ellman assay, which quantifies the hydrolysis of acetylthiocholine) [Ellman, G. L., et al. (1961), Biochem. Pharmacol. 7, 88-95], in the absence or ...
An Ellman assay (15) was used to measure formation of the thiolate dianion of 5,5′-dithiobis-(2-nitrobenzoic acid) at 412 nm (Δε412 nm = 14.15 mm−1cm−1; (16)) for 1–3 min on a Varian Cary 3A spectrophotometer, and substrate concentrations were corrected for substrate ...
Jarvinen P,Vuorela P,Fallarero A, et al.Potency determinations of acetylcholinesterase inhibitors using Ellman’’s reaction-based assay in screening:Effect of assay variants. Analytical Biochemistry . 2011Jarvinen, P.; Vuorela, P.; Hatakka, A.; Fallarero, A. Potency determinations of ...
(−log IC50) as an expression of drug potency. In various assay conditions only datasets that follow Ellman assay method were chosen because it is most common method58. Among 82 diverse compounds, 58 were in the range of 0.33–170000 nM were selected as the training set while the ...
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IL-1β determination was carried out as per the protocol provided in the assay kit (Elabscience, India). IL-1β levels were represented as pg/mg of total protein. 2.9. Mitochondrial complexes function assessment 2.9.1. Isolation of mitochondria from brain tissue The hippocampus was dissected ...