Immunoblot of human muscle biopsies was performed using the same protocol described above using the following primary antibody: rat anti-CD68 (1:500, Bio-Rad), rabbit anti-iNOS (1:2000, Abcam), rat anti-CD206 (1:500, Bio-Rad), and rabbit anti-Iba1 (1:500; Wako). Membrane preparation...
Two-electrode voltage-clamp (TEVC) measurements were performed on oocytes that heterologously expressed human α9 alone (a–c) or a combination of α9 and α10 (d–f) nicotinic acetylcholine receptor (nAChR) subunits. (a,d) Choline (Cho, 1 mM, black bars) induced repetitive stimulations ...
Donkey anti-rabbit IgG (H+L) secondary antibody, Alexa Fluor 488-conjugate Thermo Fisher Cat# A-21206, RRID:AB_2535792 Donkey anti-rabbit IgG, Cy3-conjugate Merck Millipore Cat# AP182C, RRID:AB_92588 Donkey IgG anti-rabbit IgG (H+L), Cy5-conjugate Dianova Cat# 711-175-152, RRID:AB_...