技术背景乙酰辅酶A羧化酶(Acetyl CoA Carboxylase ; ACC ; EC6.4.1.2),又称为乙酰辅酶 A碳酸氢盐连接酶(Acetyl CoA : bicarbonate ligase-ATP),是ATP依赖性含生物素酶,参与脂肪酸生物合成。哺乳动物乙酰辅酶A羧化酶单体分子量为225 - 250Kd,含有许多磷酸化位点,以及 1个生物素辅基位点、2个催化位点和1个...
a ligase that catalyzes the reaction of acetyl-CoA, CO2, H2O, and ATP, with covalently bound biotin to form malonyl-CoA, ADP, and Pi (or the reverse decarboxylase); N-carboxybiotin is an intermediate. A crucial enzyme in fatty acid synthesis. Farlex Partner Medical Dictionary © Farlex 20...
To explore the mechanism of ACC holoenzyme function, we determined the structure of the biotinoyl domain of human ACC2 and analyzed its characteristics and interaction with the biotin ligase, BirA using NMR spectroscopy. The 3D structure of the hACC2 biotinoyl domain has a similar folding topology...
Each ACC half-reaction is catalyzed by a different protein sub-complex. The vitamin biotin is covalently coupled through an amide bond to a lysine residue on biotin carboxylcarrier protein(BCCP, a homodimer of 16.7-kDa monomers encoded byaccB) by a specificenzyme, biotin–apoproteinligase(encoded...
The gene encoding biotin acetyl-CoA carboxylase ligase (BirA) from Mycobacterium tuberculosis was cloned and expressed in Escherichia coli with a C-terminal Strep-tag. PEG 4000 as well as PEG 8000 were used as precipitants at pH 7.5 to crystallize the protein using the vapour-diffusion technique...
Methods Enzymol, Acetyl-CoA carboxylase from rat liver : EC 6.4.1.2 Acetyl-CoA: carbon-dioxide ligase (ADP-forming). New York: Academic Press; 1981. p.5-16.Tanabe T, Nakanishi S, Hashimoto T, et al. (1981) Acetyl-CoA carboxylase from rat liver. Methods Enzymol 71, 5 -16....
Preparations of acetyl-CoA carboxylase [acetyl-CoA-carbon-dioxide ligase (ADP-forming), EC 6.4.1.2] have been obtained from the plastids of avocado (Persea americana) fruit mesocarp and from spinach (Spinacia oleracea) chloroplasts. Both... SB Mohan,RG Kekwick - 《Biochemical Journal》 被引量...
TRIP 12 = E3 ubiquitin-protein ligase TRIP12; TPP 1 = tripeptidyl-peptidase I; B56 = serine/threonine-protein phosphatase 2 A regulatory subunit B; dynamin = dynmin GTPase; Arp 2/3 = actin related protein 2/3 complex, subunit 5; DAGL...
Biotin carboxylase [biotin-carboxyl-carrier-protein:carbon-dioxide ligase (ADP-forming), EC 6.3.4.14c] is the enzyme mediating the first step of the acetyl... H Kondo,K Shiratsuchi,T Yoshimoto,... - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量...
别名:Acetyl Coenzyme A Carboxylase; ACAS2; AceCS; Acetate CoA ligase; Acetyl CoA synthetase;Acetyl coenzyme A synthetase cytoplasmic; Acetyl-CoA synthetase; ACS; ACSA; ACSS2; Acyl activating enzyme; Acyl CoA synthetase short chain family member 2; EC 6.2.1.1; MYH7B; ACAC; ACACB; ACC alpha...