Duin, D.A. Grahame, Tight coupling of partial reactions in the acetyl-CoA decarbonylase/synthase (ACDS) multienzyme complex from Methanosarcina thermophila: acetyl C-C bond fragmentation at the a cluster promoted by pro- tein conformational changes, J. Biol. Chem. 285 (2010) 15450-15463....
The AMP-forming acetyl-CoA synthetase is regulated by lysine acetylation both in bacteria and eukaryotes. However, the underlying mechanism is poorly understood. TheBacillus subtilisacetyltransferase AcuA and the AMP-forming acetyl-CoA synthetase AcsA form an AcuA•AcsA complex, dissociating upon lysin...
Hospital. Kaplan then joined with Sidney Colowick at the McCollum Pratt Institute at Johns Hopkins, where they shared tn the seminal discovery of the “pyridine nucleotide transhydrogenases” with Elizabeth Neufeld, who later established her reputation in the mucopolysaccharidoses (MPS) with L-id...
In an elegant study of pathway stoichiometry, free energy supply, and redox balancing, it was found that the theoretical yield of farnesene from glucose would be highest when a combination of the phosphoketolase/transacetylase reaction and an ATP-independent pyruvate to acetyl-CoA route (as ...
The acetyl-CoA decarbonylase/synthase (ACDS) multienzyme complex catalyzes the reversible cleavage and synthesis of acetyl-CoA in methanogens. This report of the enzyme complex in Archaeoglobus fulgidus demonstrates the existence of a functional ACDS complex in an organism that is not a methanogen....
Paul Balbo and Marcos Oliveira.Crystallization and preliminary X-ray data of the alpha2e2 subcomponent of the acetyl-CoA decarbonylase/synthase multienzyme complex from Methanosarcina thermophila. Acta Crystallographica . 2003Balbo P, Oliveira M. Crystallization and preliminary X-ray data of the α2ε...
The 8β88δ8ϵ8 acetyl-CoA decarbonylase synthase (ACDS) complex catalyzes the first step in the conversion of acetate to methane. The critical C-C bond cleavage of the acetyl group is mediated by the A-center, aNi-X-Fe4S4 cluster that has been localized to the β-subunit. To ...
David A. Grahame,Simonida Gencic,Edward DeMoll. A single operon-encoded form of the acetyl-CoA decarbonylase/synthase multienzyme complex responsible for synthesis and cleavage of acetyl-CoA in Methanosarcina thermophila[J]. Archives of Microbiology . 2005 (1)...
2003. Nickel in subunit beta of the acetyl- CoA decarbonylase/synthase multienzyme complex in methanogens. J. Biol. Chem. 278:6101-6110.GENCIC, S. & D.A. GRAHAME. 2003. Nickel in subunit β of the acetyl-CoA decarbonylase/synthase multienzyme complex in methanogens. J. Biol. Chem. ...
Chemically distinct Ni sites in the A-cluster in subunit beta of the Acetyl-CoA decarbonylase/synthase complex from Methanosarcina thermophila: Ni L-edge absorption and X-ray magnetic circular dichroism analyses. J Am Chem Soc. 2004; 126:88-95. [PubMed: 14709073]...