The acetyl-CoA decarbonylase/synthase (ACDS) multienzyme complex catalyzes the reversible cleavage and synthesis of acetyl-CoA in methanogens. This report of the enzyme complex in Archaeoglobus fulgidus demonstrates the existence of a functional ACDS complex in an organism that is not a methanogen....
The acetyl-CoA decarbonylase/synthase (ACDS) multienzyme complex catalyzes the reversible cleavage and synthesis of acetyl-CoA in methanogens. This report of the enzyme complex in Archaeoglobus fulgidus demonstrates the existence of a functional ACDS complex in an organism that is not a methanogen....
Hospital. Kaplan then joined with Sidney Colowick at the McCollum Pratt Institute at Johns Hopkins, where they shared tn the seminal discovery of the “pyridine nucleotide transhydrogenases” with Elizabeth Neufeld, who later established her reputation in the mucopolysaccharidoses (MPS) with L-id...
The AMP-forming acetyl-CoA synthetase is regulated by lysine acetylation both in bacteria and eukaryotes. However, the underlying mechanism is poorly understood. TheBacillus subtilisacetyltransferase AcuA and the AMP-forming acetyl-CoA synthetase AcsA form an AcuA•AcsA complex, dissociating upon lysin...
2003. Nickel in subunit β of the acetyl-CoA decarbonylase/synthase multien- zyme complex in methanogens. J. Biol. Chem. 278:6101-10Gencic S, Grahame DA (2003) Nickel in subunit beta of the acetyl-CoA decarbonylase/synthase multienzyme complex in methanogens. Catalytic properties and ...
Direct synthesis and cleavage of acetyl-CoA are carried out by the bifunctional CO dehydrogenase/acetyl-CoA synthase enzyme in anaerobic bacteria and by the acetyl-CoA decarbonylase/ synthase (ACDS) multienzyme complex in Archaea. In both systems, a nickel- and Fe/S-containing active site metal...
Chemically distinct Ni sites in the A-cluster in sub-unit尾of the acetyl-CoA decarbonylase/synthase complex from methanosarcina thermophila:Ni L-edge absorption and X-ray mag-netic circular dichroism analyses. Funk T,Gu W W,Friedrich S,Wang H X,Gencic S,Grahame D A,Cramer S P. Journal...
The 8δ8ϵ8 acetyl-CoA decarbonylase synthase (ACDS) complex catalyzes the first step in the conversion of acetate to methane. The critical C-C bond cleavage of the acetyl group is mediated by the A-center, aNi-X-Fe4S4 cluster that has been localized to the β-subunit. To elucidate...
David A. Grahame,Simonida Gencic,Edward DeMoll. A single operon-encoded form of the acetyl-CoA decarbonylase/synthase multienzyme complex responsible for synthesis and cleavage of acetyl-CoA in Methanosarcina thermophila[J]. Archives of Microbiology . 2005 (1)...
The 5-subunit-containing acetyl-CoA decarbonylase/synthase (ACDS) complex plays an important role in methanogenic Archaea that convert acetate to methane, by catalyzing the central reaction of acetate C-C bond cleavage in which acetyl-CoA serves as the acetyl donor substrate reacting at the ACDS...