易基因的研发小伙伴牢记老师们的反馈,日以继夜的奋战终于在6月之际。推出新一代羟甲基化研究科研服务产品:APOBEC-coupled epigenetic sequencing ,简称(ACE-seq)。 ACE-seq是一种全新的检测DNA羟甲基化的技术,由美国宾夕法尼亚大学Emily K Schutsky等在2018年发表在Nature Biotechnology杂志上。不同于传统的BS...
易基因的研发小伙伴牢记老师们的反馈,日以继夜的奋战终于在6月之际。推出新一代羟甲基化研究科研服务产品:APOBEC-coupled epigenetic sequencing ,简称(ACE-seq)。 ACE-seq是一种全新的检测DNA羟甲基化的技术,由美国宾夕法尼亚大学Emily K Schutsky等在2018年发表在Nature Biotechnology杂志上。不同于传统的BS...
关于ACE-seq羟甲基化测序技术 APOBEC-coupled epigenetic sequencing(ACE-seq)技术是一种全新的鉴定5hmC的方法。DNA脱氨酶APOBEC3A(A3A)能够特异地脱去C及5mC的氨基使其变为U,而5hmC则因为不被该酶识别,测序时仍被识别为C。该技术利用该酶的这种特性,特异性地检测基因组上发生的5hmC修饰。ACE-seq原理示意如下:...
利用软件Masslynx V4.1对其质谱图进行处理后,经MassEnt3转换和Peptide Sequencing软件解析出样品的氨基酸序列为WQVLPNAVPAK,软件计算的相对分子质量为1 221.992,与开始时选择的母体肽WQVLPNAVPAK序列相符,分子质量相近,如图8所示,因此可见胰...
随着羟甲基化修饰在疾病发展中的调控作用以及在基因组 DNA 中的定量定位等信息逐渐被重视,多种DNA 羟甲基化修饰检测方法也相继问世。基于亚硫酸氢盐的方法如 OxBS-seq、TAB-seq,能够在单碱基分辨率上检测 5hmC,为科学和疾病研究提供了较全面和精确的定量信息,但 OxBS-seq 还需同时构建 BS-seq文库,这种联合测序方...
Nondestructive, base-resolution sequencing of 5-hydroxymethylcytosine using a DNA deaminase 1、背景 传统的鉴定5hmC的技术需要运用化学试剂进行转化,会破坏基因组DNA,且需要的DNA起始量较大。建立一种非破坏性的、低起始DNA量的鉴定5hmC的技术有助于加快加深5hmC的研究。
here we analyzed the single-cell RNA sequencing (scRNA-seq) datasets derived from major human physiological systems including respiratory, cardiovascular, digestive and urinary systems. By interrogating the scRNA-seq data, we not only identified the organs such as lung, heart, oesophagus, kidney, bla...
A5: 这篇文章是参考谢晓亮实验团队Potent Neutralizing Antibodies against SARS-CoV-2 Identified by High-Throughput Single-Cell Sequencing of Convalescent Patients’ B Cells这篇文献，发表于cell上，这图片展示的是噬菌斑减少中和试验(Plaque reduction neutralization test PRNT)结果，用于检测中和抗体的中和效价。不...
The chromosome 6 database (6ace) is one of a suite of databases available at the Sanger Centre, which serve the human genome sequencing communities of several chromosomes (1, 6, 20, 22, and X). Data may be retrieved in graphical or textural form using interactive windows and menus or ...
(2002) 16S ribosomal DNA sequencing confirms the synonymy of Vibrio harveyi and V. carchariae. Diseases of Aquatic Organisms 52, 39–46. Article PubMed CAS Google Scholar Gauger, E., Smolowitz, R., Uhlinger, K., Casey, J. and Gómez-Chiarri, M. (2006) Vibrio harveyi and other ...