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All other reagents and solvents were sourced from VWR, Avantor (Radnor, PA) or Sigma-Aldrich, Millipore Sigma (St. Louis, MO) without any further purification unless otherwise indicated. Molecular self-assembly and co-assembly of PAs to form supramolecular structures For self-assembly of PAs, ...
The evolutionary origin and historical demography of extant Arcto-Tertiary forest species in East Asia is still poorly understood. Here, we reconstructed the evolutionary and population demographic history of the two extant Euptelea species in China (E.
For ex vivo stimulation assays analyzed by CyTOF, 250 µL of heparinized whole blood cells from CPT tubes were transferred to a 5 mL round-bottom tube with a lid (Thermo Fisher Scientific, Waltham, MA), followed by addition of RPMI medium (Sigma Aldrich, St. Louis, MO), resiquimod...
Polybrene (TR-1003-G, Sigma Aldrich, St. Louis, MO, USA) was added to a final concentration of 5 μg/mL. 60 h post-infection, cells were analyzed by flow cytometry. Titers were calculated using the Poisson formula. If P is the percentage of cells that are ZsGreen positive, as ...
(Abbott Lab, Germany) concentration in inspired air. Standard echocardiograms were recorded by a person blinded to the experiment and analyzed with Echo PAC software (GE Medical Systems CZ Ltd., Prague, CZ). Directly measured parameters from M-mode included left ventricle end-diastolic anterior ...
We would also like to thank the Boston University Medical Campus Alumni Library, the Boston University School of Medicine Cellular Imaging Core and everyone in the Finland Laboratory for Pediatric Infectious Disease at Boston Medical Center, particularly lab manager Yazdan Dasthagrasaheb for all of ...
For THP-1 monocyte to macrophage differentiation, THP-1 cells were stimulated with 100 nM PMA (Sigma-Aldrich, St. Louis, MO, USA) for 48 h. Human monocyte-derived macrophages (MDMs) were derived from CD14+ peripheral blood monocytes by culturing cells in RPMI/1640 media containing 10% heat...