ACC-deaminase producing rhizobacteria are responsible in facilitating the resistance to plants against stressful environmental conditions including salinity, drought, flooding, metal and organic contamination, and phytopathogens. ACC-deaminase is an inducible enzyme and its synthesis is stimulated in the ...
往预先包被ACC 脱氨酶(ACC deaminase)抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成终的黄色。颜色的深浅和样品中的ACC 脱氨酶(ACC deaminase)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算...
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In this study ACC deaminase from iIssatchenkia occidentalis /iwas isolated, partially purified and characterized. 1-Aminocyclopropane-1-carboxylic acid (ACC) deaminase catalyses the conversation of ACC to α-ketobutyric acid and ammonia. Most of the studies on this enzyme were done mainly from ...
从阴沟肠杆菌(Enterobacter cloacae)UW4菌株克隆出ACC脱氨酶(1-aminocyclopropane-1-carboxylicacid deaminase)基因连接至pGEM-Tvector上,并采用3种不同启动子(组成型表达启动子CaMV35S、花特异表达启动子CHSA及衰老特异表达启动子SAG12)分别构建ACC脱氨酶基因的植物表达载体,经PCR及酶切鉴定均已构建成功,为后期选择不同...
Rhizobacteria capable of producing ACC-deaminase may mitigate salt stress in wheat. Soil Science Society of America Journal. 2010;74(2):533-42.Nadeem SM, Zahir ZA, Naveed M, Asghar HN, Arshad M (2010a) Rhizobacteria capable of producing ACC-deaminase may mitigate salt stress in wheat. Soil...
Some strains of soil bacteria were isolated by their ability to grow on ACC as a sole nitrogen source.ACC deaminase activity of these strains were determined by growth assaying,confirmed by chromatograthically identifying to decrease of ACC in the medium.Then systematic identification was done.Wei ...
通过根癌农杆菌(Agrobacterium tume faciens)介导法将色氨酸单氧化酶(iaaM)基因和1-氨基环丙烷-1-羧酸(ACC)脱氨基酶基因转入矮牵牛,重金属抗性实验表明,表达iaaM基因的矮牵牛茎生长得到促进,生长速度快,但对钴毒害较为敏感;共同表达iaaM和ACC脱氨基酶基因的矮牵牛不但生长快,而且对钴毒害有较强的耐受性,在钴浓度...
The bacterium has an enzyme 1-Aminocyclopropane-1-carboxylate deaminase that intermediates with ethylene synthesis pathway and regulates ethylene accumulation, which, in turn, reduces the damage caused by environmental stresses. Thus, a comprehensive review of the role of rhizobacterial ACC deaminase in ...
ACC deaminase is an inducible enzyme whose synthesis is induced in the presence of its substrate ACC. This enzyme encoded by gene AcdS is under tight regulation and regulated differentially under different environmental conditions. Regulatory elements of gene AcdS are comprised of the regulatory gene...