Oncotype DX29panel was built (Supplementary Note5). Expression of each gene is calculated from the molecule count of each amplicon, based on UMI count and conversion yield, and is further normalized relative to the expression level of the 5 reference genes in log2 scale (Supplementary Fig.S9)...
The details of gene-specific qRT-PCR primers are listed in Supplementary Table S4. The primers were designed using the Primer-BLAST program (https://www.ncbi.nlm.nih.gov/ tools/primer-blast) based on National Center for Biotechnology Information (NCBI) sequence data (http://www.ncbi.nlm....