This is particularly important when the recommended agent is a new and/or infrequently employed drug.Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance ...
In order to examine the expression of HORMAD1 in cancers thoroughly, we conducted pan-cancer analysis of HORMAD1 expression in 25 types of cancers using RNA sequencing data from The Cancer Genome Atlas (TCGA) (Fig. 1a). In physiological conditions, HORMAD1 expression is restricted to meiotic...
In vivo tumorigenesis assay was conducted to evaluate the effect of MAGE-A3 on tumor growth. Results MAGE-A3 expression was significantly up-regulated in CC cell lines (HeLa, SiHa and C33A) compared with that in End1/E6E7 cell line. Knockdown of MAGE-A3 could significantly suppress migration...
Limma package in R was conducted to analyze abnormally expressed lncRNAs (differentially expressed lncRNAs [DELs]) and mRNAs (differentially expressed genes). The prognostic roles of screened DELs in HCC were evaluated at GEPIA. The expression of DELs that correlated with overall survival of HCC ...
conducted ethically in accordance with the World Medical Association Declaration of Helsinki and approved by the local ethical review committee of the First Affiliated Hospital of Guangzhou University of Chinese Medicine (no. ZYYECK[2017]069). The clinical trial was registered in a Chinese Clinical ...
To confirm that azathioprine downregulates lipid/cholesterol metabolism in GBM, we first conducted western blot analysis to assess expression of proteins differentially expressed following azathioprine treatment. Decreased LDLR, MSMO1, FDFT1, and FADS2 protein expression was observed in azathioprine-treated...
Finally, to appraise aberrant signaling in tumor cells, we conducted a comparative analysis of the total count and strength of cell–cell interactions between tumor cells and epithelial cells. Single-cell transcription factor analysis We used the SCENIC R package (version 1.3.1) (https://github....
Further, qRT-PCR and luciferase reporter assays were conducted to examine the regulatory role of miR-193a controlling the HOTAIR expression in PCa. Finally, the correlation between HOTAIR, EZH2 and miR-193a expression were analyzed using In situ hybridization and immunohistochemistry. Results We ...
The enzymatic reaction was conducted with 1-Step Ultra TMB-ELISA. The optical density was measured at 655 nm using an iMark microplate reader. These reactions were performed in a volume of 50 ml at 37uC. The error bars show the standard deviation of three independent experiments. (b) ...
and J.S. conducted the main experiments and analyzed the data. J.S. completed the immunofluorescence experiments. J.Liu, K.W. and Y.Q. completed the animal experiment. H.L. completed the bioinformatics analysis. Y.W. and X.Zhang helped to complete the molecular cloning experiment. K.W....