Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer)Human tonsil tissue incubated with ab16667 overnight at +4°C.Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer pH 6.0).Nuclear staining on human tonsil. The...
Despite its simple protocol, the Lightning-Link® process quickly generates reproducible conjugates with no loss of antibody, saving you valuable time and resources. It can also be used to conjugate other proteins or peptides. Learn more about the advantages of our antibody labeling kits....
VeriBlot for IP secondary antibodies, which preferentially detect the non-reduced, non- denatured primary antibody during western blotting. 100 mM EDTA stock solution is made with 1.86 g EDTA dissolved into 40 ml H2O. Add NaOH to adjust the pH to 7.4. Finally, adjust the total volume to ...
DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 M.The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.This product also gave a positive signal under the same testing conditions in HeLa cells fixed ...
Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step. When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin...
using 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 25°C; followed by incubation with the primary antibody, at a 1/500 dilution, for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor®488 used at a 1/5000 dilution...
Anti-MannoseReceptorantibody(ab64693)at1µg/ml+ Liver(Rat)TissueLysateat10µg Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Performed under reducing conditions. Predicted band size : 166 kDa
What temperature should I store the antibody at? Storage at 4°C should not exceed one or two weeks. Upon arrival, briefly spin the antibody tube to pull down the solution and aliquot the secondary antibody solution. Aliquots should be stored at -20°C or -80°C. ...
Secondary antibody (goat anti-rabbit IgG-HRP,ab97051, or goat anti-mouse IgG-HRP) ECL substrate Streptavidin-AlexaFluor® 488 Methylene blue staining buffer (0.2% Methylene blue in 0.4M sodium acetate and 0.4M acetic acid) Equipment ...
(Human monocytic leukemia monocyte) labeling CD4 with purified ab133616 at 1/100 dilution. Cells were fixed with 100% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] -...