Secondary All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366) at 1/5000 dilution Predicted band size: 76 kDa Observed band size: 76 kDa Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) All lanes : Anti-Rb (phospho T373) antibody [EP821...
Incubate the membrane with a secondary antibody. 30 min at room temperature Different secondary antibody dilutions could be used depending on the batch difference. We dilute our secondary antibody 1:50 000 in 10 mL of the blocking buffer. 5 Wash the membrane three times with TBST. 10 min per...
using 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 25°C; followed by incubation with the primary antibody, at a 1/500 dilution, for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor®488 used at a 1/5000 dilution...
a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counter
The company provides a range of non-primary antibody products such as secondary antibodies, kits, proteins, peptides, and lysates. It was founded in 1998 and is based in Cambridge, United Kingdom. In December 2023, Abcam was acquired by Danaher.7B. Headquarters Location Discovery Drive ...
antibody(1/100in1xPBS+10%heatinactivatedFCS)for 1hour.AnundilutedHRP-conjugatedRabbitanti-rabbit IgGpolyclonalwasusedasthesecondaryantibody. Westernblot-MannoseReceptorantibody(ab64693) Anti-MannoseReceptorantibody(ab64693)at1µg/ml+ Liver(Rat)TissueLysateat10µg Secondary Goat polyclonal to Rabbit IgG...
24°C Samples were incubated with primary antibody(1/500 in PBS)for 16 hoursat4°C An Alexa Fluor®555-conjugated Rabbit anti-goat IgG polyclonal (1/1000)was used as the secondary antibody IHC-P image of Iba1 staining on cat kidney sections using ab5076(1 :1000)The sections were depa...
To pick your secondary antibody conjugated to gold nanoparticles, filter your results based upon nanoparticle size, species reactivity plus additional options. How does the antibody or biomolecule bind to gold? Antibodies or biomolecules attach covalently to a 'conjugation 'friendly' coatingon gold nanop...
A clone number is given to an antibody produced by a single clone of hybridoma cells. Polyethylene glycol (PEG): what do I need to know? Answers to your frequently asked questions on PEG. Cellular and biochemical assay kits: what general information do I need to know?
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). ChIP - Anti-beta Catenin antibody [E247] (ab32572) Chromatin was prepared from HCT 116 cells according to the Abcam Dual-X-ChIP protocol*. Cells ...