Intraperitoneal AAV9-shRNA inhibits target expression in neonatal skeletal and cardiac muscles. Biochem Biophys Res Commun. 2011; 405(2):204-209. [PubMed: 21219850]Mayra A, Tomimitsu H, Kubodera T, et al. Intraperitoneal AAV9- shRNA inhibits target expression in neonatal skeletal and cardiac...
past reports are related to the expression of a reporter or functional protein; there have been no reports demonstrating silencing of a target gene in the nervous system via intraperitoneal injection. In the present study, we injected single-stranded AAV9-shRNA (ssAAV9-shRNA) intraperitoneally to...
2.SHR心肌转染的效应:(1) Real-time RT-PCR结果显示,AAV9-shRNA-ADRB1-ZsGreen注射后β1-AR mRNA表达明显低于阴性对照组(0.4142±0.3399vs.1.0933±0.5853,P0.05);(2)免疫组化结果显示,AAV9-shRNA-ADRB1-ZsGreen注射后β1-AR蛋白表达明显低于阴性对照组及假手术组(50.02±4.44vs.80.05±3.72vs.79.30±2.79...
In this unit, we describe protocols for use of rAAVs as a cargo to deliver miRNA backbone‐based shRNA controlled by a cardiac‐specific promoter into the mouse heart. © 2016 by John Wiley & Sons, Inc.doi:10.1002/cpmb.9Hiroko Wakimoto...
Intraperitoneal administration of AAV9-shRNA inhibits target gene expression in the dorsal root ganglia of neonatal mice 町田 明 被引量: 0发表: 2013年 Aerobic exercise mitigates high-fat diet-induced cardiac dysfunction, pyroptosis, and inflammation by inhibiting STING-NLRP3 signaling pathway ...
In this unit, we describe protocols for use of rAAVs as a cargo to deliver miRNA backbone‐based shRNA controlled by a cardiac‐specific promoter into the mouse heart. © 2016 by John Wiley & Sons, Inc.doi:10.1002/cpmb.9Hiroko Wakimoto...
Systemic delivery of sh RNA by AAV9 provides highly efficient knockdown of ubiquitously expressed GFP in mouse heart,but not liver. Piras BA,O’’Connor DM,French BA. PLo S One . 2013Piras BA, O'Connor DM, French BA. Systemic delivery of shrna by aav9 provides highly efficient knockdown...
GW27-e0227 Downregulation of the β1 adrenergic receptor in myocardium by AAV9-shRNA-ADRB1-ZsGreen injection reduce blood pressure in spontaneously hypertensive ratsGaiyanWenYuanHongingentaconnectJournal American College of Cardiology
GW27-e0227 Downregulation of the β1 adrenergic receptor in myocardium by AAV9-shRNA-ADRB1-ZsGreen injection reduce blood pressure in spontaneously hypertensive ratsThis paper describes a new portable real-time remote electrocardiography (ECG) monitor based on mobile communication technology. ...
AAV9 Delivery of shRNA to the Mouse Heart: AAV9 Delivery of shRNA to the Mouse Heart RNA interference (RNAi) is a rapid approach to dissect loss‐of‐function phenotype for a gene of interest. However, it is challenging to perform RNAi in s... H Wakimoto,JG Seidman,RSY Foo,... -...