The use of cell-type specific promoters such as human synapsin (hSYN) can restrict transgene expression profiles to specific cell types or tissues [63, 64]. Other studies suggest that promoter-capsid interactions can also influence in vivo expression [65, 66]. Future studies designed to pair ...
Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nat Biotechnol. 2016;34:204–9. 42. Sack BK, Herzog RW. Evading the immune response upon in vivo gene therapy with viral vectors. Curr Opin Mol Ther. 2009;11:493–503. 43. Wang D, Tai PWL, ...
31 By driving expression of the GFP reporter gene under a fragment of the murine truncated GFA promoter or a human synapsin promoter, we successfully restricted expression to astrocytes or neurons in the brain after AAV9 intravenous injection. Of importance, no GFP-positive hepatocytes could be ...