ssrAAV9-CMV-EGFP转导试剂盒主要针对实验细胞和动物进行EGFP外源基因的转导与表达研究,产品具有独特的安全性、可靠性和稳定性,特别适合对基因表达载体要求高的基础研究或临床试验。 产品采用最为安全和经典的三质粒共转染技术,以最常用的CMV为外源基因启动子、最便于检测的EGFP作为外源标记基因、具中枢神经靶向性的AAV9...
1.2.3 pAAV-ITR-CMV-AAV9-HGF 病毒滴度测定 取6 μL 纯化后的重组腺相关病毒,去除DNA 污染并裂解病毒得到其基因组DNA。然后进行qRT-PCR 检测,检测对象为腺相关病毒载体上的WPRE 序列,上游引物为F:5’- CCGTTTCA GGCAACGTG-3’;R:5’-AGCTGACAGGTGGTGGCAAT-3’。pAAV-EGFP 质粒按10、102、103、104和...
CAG启动子保留了CMV增强子,使其具有很强的转录能力,而其中的鸡β-肌动蛋白启动子则使其具有更加广泛和广谱的表达能力。腺相关病毒是Dependovirus属Parvovirus家族的微小单链DNA病毒,包含100多种血清型。其所介导的基因转移系统可整合至基因组中从而维持外源基因长期稳定表达,广泛用于基因治疗、动物体内目的基因的过表达...
egfp载体的mcs多克隆位点插入人sphk2基因cds序列nm_001204159,构建得到腺相关病毒sphk2。 82.3、实验方法及试验结果 83.a.对照组(aav9 ‑ gfp mi):对需要构建小鼠心肌梗死模型的小鼠,在其出生后第3天用微量进样针,吸取50μl aav9 ‑ ctnt ‑ gfp病毒(滴度为1*10 12 vg/ml),于左心室原位注射。 84....
[0051]EGFP是编码修饰绿色荧光蛋白的序列, 多聚A是多聚腺苷酸化信号, ITR是腺相关病毒的反向末端重复, HBG内含子是人β‑珠蛋白内含子, CMV启动子是人巨细胞病毒启动子, AmpR是β‑内酰胺酶基因的序列,其提供大肠杆菌对氨苄青霉素的抗性, pUC起点是高拷贝细菌复制起点。 [0052]图3是旨在用于从随机变体文库...
AAV9 has significantly lower seroprevalence in the human population than other AAV serotypes, making AAV9 a desirable candidate for therapeutic applications.These AAV9 particles constitutively express ZsGreen under a CMV promoter. ZsGreen is a human codon-optimized variant of the green fluorescent ...
AAV9 vector (1e+11 viral genome particles/100 渭l), encoding firefly luciferase (AAVCMVLuc) or eGFP (AAVCMVeGFP) under the control of CMV promoter, was infused into the left kidney via the ureter and held for a period of 15 minutes. Non-invasive bioluminescence imaging (IVIS-200, ...
The first randomly calculated combination yielding equal group sizes was Generation of AAV vectors Murine desmin cDNA was inserted with BamHI/BsrGI into a self- complementary AAV vector genome plasmid, derived from pdsCMV-MLC0.26- EGFP,29 under control of the human troponin T promoter (hTNNT2),...
The first randomly calculated combination yielding equal group sizes was Generation of AAV vectors Murine desmin cDNA was inserted with BamHI/BsrGI into a self- complementary AAV vector genome plasmid, derived from pdsCMV-MLC0.26- EGFP,29 under control of the human troponin T promoter (hTNNT2),...
Given the impossibility to detect hNDUFS4 protein by immunofluorescence, we used an AAV2/9-CMV-eGFP vector as a proxy to evaluate the distribution of the viral vector in the brain tissue. Immunofluorescence analysis of coronal brain sections from wild-type mice ICV-injected with 2 × 1010vg ...