AAV-CAG-Cre-GFP (AAV9),即AAV9 serotype adeno-associated virus (AAV) expressing Cre-GFP,是一种可表达Cre和GFP融合蛋白的腺相关病毒,常用于注射小鼠等动物后进行基因编辑。CAG启动子是一种由巨细胞病毒(CMV)增强子和鸡β-肌动蛋白启动子组合而成的可以用于大多数基因编辑的高效启动子,驱动基因在真核细胞内...
Intraplacental injection of AAV9-CMV-iCre results in the widespread transduction of multiple organs in double-reporter mouse embryosdoi:10.1538/expanim.23-0044GOGOLEVA, NataliaSHAHRI, Zeynab JavanfekrAtsushi NODAChing-Wei LIAOArata WAKIMOTOYuri INOUE...
In order to develop a suitable vector for gene therapy in Leigh disease, a multisystem mitochondrial disease, we cloned the human wild-typeNDUFS4cDNA (hNDUFS4) into a single-stranded AAV2/9 viral vector under the control of the strong, general promoter of the cytomegalovirus (CMV) (AAV2/...
Given the impossibility to detect hNDUFS4 protein by immunofluorescence, we used an AAV2/9-CMV-eGFP vector as a proxy to evaluate the distribution of the viral vector in the brain tissue. Immunofluorescence analysis of coronal brain sections from wild-type mice ICV-injected with 2 × 1010 vg...
A 700-bp mouse DNA fragment encompassing Mir34a was subcloned from the pENTR/D-TOPO-Mir34a plasmid43 into the scAAV9-CMV plasmid. This scAAV9-MiR34a construct was first transfected into mouse MiR34a-KO ESCs to verify expression and then it was packaged by the AAV Core Facility in Academi...
We hypothesized that, when compared to the ubiquitously expressed hybrid CMV/CBA promoter element, both scAAV9-GFA-GFP and scAAV9-Syn-GFP would lead to targeted GFP expression of in specific sub-populations of the brain (respectively astrocytes or neurons), while greatly decreasing peripheral ...
AAV generation A flag-tagged (N-terminal) open reading frame of mouse Rbm24 (ENSMUST00000037923;NCBIm37) was cloned into the pZac2.1 vector (under control of a CMV-promotor) and was subsequently used for AAV generation. AAVs were generated by the AAV Vector Unit at ICGEB Trieste (http:/...
To first test the efficacy of dual vector delivery with AAV9-PHP.B, we generated AAV2/9-PHP.B-CMV-turbo red fluorescent protein (RFP)-WPRE (PHP.B-RFP) and coinjected this vector with AAV2/9-PHP.B-CMV-EGFP-WPRE at a 1:1 gc ratio in a total volume of 1 μL into the utricle...