ssrAAV9-CAG-EGFP转导试剂盒主要针对实验细胞和动物进行EGFP外源基因的转导与表达研究,产品具有独特的安全性、可靠性和稳定性,特别适合对基因表达载体要求高的基础研究或临床试验。 产品采用最为安全和经典的三质粒共转染技术,以常用的CAG为外源基因启动子、最便于检测的EGFP作为外源标记基因、具中枢神经靶向性的AAV9为...
因此,本产品适合用于小鼠等动物的体内感染,不太适合用于培养细胞的感染。如果用于培养细胞的基因编辑,推荐选购Lenti-EF1α-Cre-P2A-EGFP-Puro (10^8TU/ml) (C4102)。如果希望使用本产品之外的其它血清型的AAV-CAG-Cre-GFP,可以联系碧云天的技术服务部进行定制。
为了进一步验证变体体内感染效果并探究感染的细胞类型,包装EGFP病毒,玻璃体腔注射小鼠(3E+9vg/只眼),3W后通过活荧光眼底拍照检测EGFP整体表达情况,摘取眼球进行病理检测。眼底拍照结果(图12)显示,PM054的GFP信号最强,3种变体荧光信号均显著高于AAV2和AAV2.7M8。
基因治疗正日益成为罕见病乃至单基因遗传病的首选治疗策略,提升基因药物的体内表达是重要研究领域.本文选择4种短内含子构建了4种嵌合型启动子CAR,CAS,CAP,CAT,通过Gluc报告基因表达活性比较发现,CAR强度明显高于CA,仅次于CAG.选择CAR启动子构建和制备了携带EGFP报告基因的重组病毒rAAV9-CAR-EGFP,经SDS-PAGE分析可见...
(N = 6 per condition). After 3 weeks, mice were re-administered 1 × 1012v.g. per animal PHP.eB or AAV-X1.1 packaging CAG-eGFP (N = 3 per condition). Mice were randomly assigned to a particular AAV condition. Experimenters were not blinded for any of the experiments ...
clinical trials for treating neurological disorders are its high expression in many off-target tissues such as liver and heart, and lack of cell specificity within the central nervous system (CNS) when using ubiquitous promoters such as human cytomegalovirus (CMV) or chicken-β-actin hybrid (CAG)...
1 材料与方法 1.1 材料 1.1.1 病毒与细胞 AAV9-EGFP(物理滴度为1.00×1012?vg/mL)、AAV9-101(物理滴度为2.75×1013?vg/mL)载体制品由北京五加和基因科技有限公司制备;HSV1-rc/△UL2(简称HSV1-rc)是将AAV2?rep-cap基因置于人HSV1基因组中构建的重组病毒[6,7],毒种由北京五加和基因科技有限公司保存。
We therefore injected 1 × 1011 vector genomes (vg) each of single-stranded AAV9 (ssAAV9)-CB7-EGFP and ssAAV-PHP.eB-CAG-tdTomato into the lateral tail vein of adult B6C3 mice, and analyzed GFP and tdTomato expression in the brain 30 days post-injection. Unlike in the C57BL/6J ...