AAV-CAG-Cre-GFP (AAV9),即AAV9 serotype adeno-associated virus (AAV) expressing Cre-GFP,是一种可表达Cre和GFP融合蛋白的腺相关病毒,常用于注射小鼠等动物后进行基因编辑。CAG启动子是一种由巨细胞病毒(CMV)增强子和鸡β-肌动蛋白启动子组合而成的可以用于大多数基因编辑的高效启动子,驱动基因在真核细胞内...
Rapid and highly efficient inducible cardiac gene knockout in adult mice using AAV-mediated expression of Cre recombinase. Cardiovasc Res 2014; 104: 15–23. 22 van Putten M, van der Pijl EM, Hulsker M, Verhaart IEC, Nadarajah VD, van der Weerd L et al. Low dystrophin levels in heart ...
Rapid and highly efficient inducible cardiac gene knockout in adult mice using AAV-mediated expression of Cre recombinase. Cardiovasc Res 2014; 104: 15–23. 22 van Putten M, van der Pijl EM, Hulsker M, Verhaart IEC, Nadarajah VD, van der Weerd L et al. Low dystrophin levels in heart ...
Fig. 5: LRP6 modulates CNS function of engineered AAVs in mice. aSchematic ofLrp6conditional knockout by sequential AAV injection. Cre-conditionalLrp6knockout mice were systemically injected with AAV1-X1 packaging either Cre or mCherry, creating cohorts of mice that differ in theirLrp6expression....
We therefore injected 1 × 1011 vector genomes (vg) each of single-stranded AAV9 (ssAAV9)-CB7-EGFP and ssAAV-PHP.eB-CAG-tdTomato into the lateral tail vein of adult B6C3 mice, and analyzed GFP and tdTomato expression in the brain 30 days post-injection. Unlike in the C57BL/6J ...