GENEWIZ from Azenta’s proprietary Sanger sequencing method sequences through difficult inverted terminal repeat (ITR) regions of adeno-associated virus (AAV), to expedite screening and validation of leads for your cell and gene therapy research. This newly-developed protocol prevents abrupt reduction...
为了解决这一难题,GENEWIZ开发了一种新型的ITR测序方法,可以改善ITR区域的测序信号并延长读取长度。 为了验证我们的ITR测序方法,将其与标准方法进行比较,我们首先使用BigDye®cycle sequencing kit(Thermo Fisher)对含有AAV2 ITRs的质粒进行测序。不出所料,...
At GenScript, we overcome this challenge with our AAV ITR Guarantee Service. With over 20 years of expertise, we combine a specialized ITR plasmid DNA preparation protocol with proprietary ITR Sanger sequencing technology to ensure the highest accuracy and integrity of your ITR sequences. Bundle ou...
Adeno-associated virus genome population sequencing achieves full vector genome resolution and reveals human-vector chimeras. Molecular therapy. Meth Clin Dev. 2018;9:130–41. Article CAS Google Scholar Xiao W, Chirmule N, Berta SC, McCullough B, Gao G, Wilson JM. Gene therapy vectors based...
图2:使用BigDye®cycle sequencing kit对AAV2的ITR区域进行测序 红色下划线标记序列后的碱基“TTGGC”是一个无法成功测序的125 bp ITR发夹结构的开端。 而GENEWIZ的新型AAV-ITR测序方法可以测通ITR发夹结构并且在整个ITR区域没有明显的信号损失,达到了与常规测序反应类似的读取长度(图3)。此外,我们还研究了引物结合...
GENEWIZ AAV-ITR Sanger Sequencing sequences difficult inverted terminal repeat (ITR) regions of adeno-associated virus (AAV), to expedite screening and validation of leads.