实验结果表明,GFAP微型启动子介导的AAV泄露表达并非是由于高病毒滴度所引起的, 而是由其携带表达的转化因子所决定 (与2021年Cell文章在大脑中的观察结果相类似);相反,使用低病毒滴度(1011-1012GC/mL)无法消除泄露表达,却会造成感染效率的急剧下降;当使用不同血清型的AAV通过不同注射途径去感染Müller胶质细胞, 也无...
Transduction efficiency, transgene expression and cellular tropism were compared for the AAV serotypes AAV5, AAV9 and AAVRec2 whereby destabilised yellow fluorescent protein (dYFP) was controlled by the GFAP or the truncated GfaABC1D promoter. The vectors were tested in primary spinal cord ...
CNTF-induced GFP expression in AAV2/6-GFAP-GFP transduced Müller glial cells.Wendy M. AartsenKoen W. R. van CleefLucie P. PellissierRobert M. HoekRogier M. VosBas BlitsErich M. E. EhlertKamaljit S. BalagganRobin R. AliJoost Verhaagen...