Expression in limbus and supporting cells was assessed using an eGFP scoring system detailed in Supplementary Fig. 2. SGN transduction was evaluated by eGFP-positive cell counts per microscopic field. Mean indicated by horizontal bar. Each condition had minimally N=3 for the 48h, and N=2 for ...
AAV1-EF1a-DIO-Flp (1.5 × 1014 GC/mL, custom design, ViGene Biosciences) was injected into V1 (60 nL) of Vglut2-Cre (Slc17a6tm2(cre)Lowl, Jackson Laboratories, RRID: IMSR_JAX:016963) or GAD2-Cre (Gad2tm2(cre)Zjh, Jackson Laboratories, RRID: MGI:4418723) mice crossed with Ai...
The AAV2.7m8-CAG-eGFP (9.75 × 1012 GC/mL), AAV8BP2-CAG-eGFP (1.10 × 1013 GC/mL), AAV2-CAG-eGFP (5.69 × 1012 GC/mL), AAV2/8-CAG-eGFP (1.66 × 1013 GC/mL), and Anc80L65-CAG-eGFP (1.89 × 1013 GC/mL...
(group means ± SEMs) for each group, as seen immediately after the cochlear function measures in (A,B). Group sizes for (A,B) were: Normal = 5; AAV-EGFP = 5. Group sizes for (C,D) were the same except Normal = 3. Symbol key in (D) applies to all panels...
Hevin基因敲除小鼠(Hevin KO)在出生后的前三周和成年后都表现出明显的谷氨酸偶体转运蛋白2阳性(VGluT2+)丘脑皮质突触的缺失(Figure 4E)。为了确定脑内皮细胞异位表达Hevin是否可以挽救Hevin-KO小鼠的缺陷,作者使用AAV9-X1.1-CAG-Hevin-EGFP的载体,尾静脉注射Hevin-KO小鼠,3周后,结果显示:在脑内皮细胞中,Hevin...
并且使用该rAAV基因组生成AAV病毒文库,然后将AAV病毒文库以1x1011vg/小鼠的剂量注射到成年的GFAP-Cre小鼠、Vgat-IRES-Cre小鼠和Vglut-IRES-Cre小鼠中,用于筛选能够分别转导星形胶质细胞、GAGA能神经元(GABAergic neurons)和谷氨酸能神经元的变体。在两轮筛选以后,发现许多变体富集在从一种或多种Cre细胞系中回收的...
Hevin基因敲除小鼠(Hevin KO)在出生后的前三周和成年后都表现出明显的谷氨酸偶体转运蛋白2阳性(VGluT2+)丘脑皮质突触的缺失(Figure 4E)。为了确定脑内皮细胞异位表达Hevin是否可以挽救Hevin-KO小鼠的缺陷,作者使用AAV9-X1.1-CAG-Hevin-EGFP的载体,尾静脉注射Hevin-KO小鼠,3周后,结果显示:在脑内皮细胞中,Hevin...
并且使用该rAAV基因组生成AAV病毒文库,然后将AAV病毒文库以1x1011vg/小鼠的剂量注射到成年的GFAP-Cre小鼠、Vgat-IRES-Cre小鼠和Vglut-IRES-Cre小鼠中,用于筛选能够分别转导星形胶质细胞、GAGA能神经元(GABAergic neurons)和谷氨酸能神经元的变体。在两轮筛选以后,发现许多变体富集在从一种或多种Cre细胞系中回收的...