AAV Production Everywhere: A Simple, Fast, and Reliable Protocol for In‐house AAV Vector Production Based on Chloroform ExtractionAAV vectoradeno associated viruschloroform extractiongene expressiongene therapyPEI transfectionvector productionRecombinant adeno-associated virus (rAAV) is a mammalian virus ...
Neither production method, at a titer of 1 × 1012 genome copies/mL (gc/mL), did result in any obvious inflammatory response nor loss of TH immunoreactive (TH-ir) neurons in the substantia nigra, at the timepoint assessed. These results combined suggest that chloroform-based AAV vector...
Production Everywhere:A Simple,Fast,and Reliable Protocol for In‑house AAV Vector Production Based on Chloroform Extraction》。 [0119]复制缺陷重组AAV可通过三质粒共转进被人类辅助病毒(例如腺病毒)感染的细胞 系而制备:所含的所关注核酸序列的侧翼为两个AAV反向末端重复序列(ITR)区域的质粒, 辅助包装质粒...