1. ConVERGD系统的开发与验证:研究团队首先成功开发了ConVERGD系统,该系统融合了自切割核糖核酸酶和FLEx开关,能够在重组酶(如Cre和Flp)的作用下表达特定的分子载荷。通过体外细胞实验,验证了ConVERGD系统在不同条件下表达的特异性。 2. ConVERGD系统的扩展:为了增强系统的多特征交叉靶向能力,研究团队对ConVERGD系统进...
AAV2/1-hSyn-Flp、 AAV2/9-Cre/Flp-NpHR-EYFP、AAV2/9-Cre/Flp-ChR2-EYFP、AAV2/9-Cre/Flp-EYFP 、AAV2/9-Cre/Flp-EYFP、 AAV2/9-Cre/Flp-EYFP 、Retro-AAV2/2-CAG-Flex-Flp 、AAV2/9-fDIO-GCaMP6s 、AAV2/9-DIO-taCasp3-TEVp、AAV2/9-hSyn-Cre、AAV2/9-DIO-taCasp3-TEVp、AAV2/...
本节将重点介绍这样一种策略- - FLEX开关,它利用重组关闭一个基因的表达,同时开启另一个基因的表达。 什么是FLEX开关 FLEX (或"翻转-切除")开关被设计为一种基因工具,研究人员利用位点特异性重组在体内有条件地操纵基因表达。FLEX开关利用了位点特异性重组酶(SSRs) Cre和FLP的方向特异性。SSRs在靶位点结合DNA,诱...
3.Cre-on,Flex,DIO,double floxed :使用 Cre-Loxp 系统,经过两次染色体重组在特定脏器中表达外源基因。ssAAV & scAAV目前,研究人员常用的两类AAV分别为single-stranded AAV (ssAAV) and self-complementary AAV (scAAV)。scAAV是基于2个基础被开发的:一个是理论基础:无论scAAV还是rAAV,病毒颗粒中均可包裹二倍...
AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA catalog : 65454 more info or order: Addgene product webpage citations: 1 Reference Madisen L, Garner A, Shimaoka D, Chuong A, Klapoetke N, Li L,et al. Transgenic mice for intersectional targeting of neural sensors and effectors with...
Data points represent independent transfections with the following constructs: 56 (FLEx(FRT)eGFP alone or +pDIRE), 6 (CV-eGFP-W3SL +Flp, +pDIRE; CV-eGFP-WPRE, +Flp, +pDire; Ef1a-CV-eGFP-WPRE, +pDIRE) 5 (INTRSECT-EYFP, +Cre, +Flp, +pDIRE; CV-eGFP-W3SL, +Cre; CV-eGFP-...
(A) Left, AAV1-hSyn-Cre was injected into V1 of Ai14 mice, followed by a second injection of AAV1-CAG-FLEX-GFP into SC. Middle, GFP-labeled neurons in SC-sg were also tdTomato+. Right four panels, GFP-labeled axons in various regions (LP, pretectal area, PBG, and cuneiform nu...
Quantitative real-time PCR was performed using PowerUp SYBR Green Master Mix in QuantStudio™ 6 7 Flex Real-Time PCR System (Life Technologies, Thermo Fisher Scientific). Human and murine SGPL1 mRNA and levels of cytokines were measured. Cq values of samples were normalized to the ...
pAAV-CAG-FLEX-EGFP-WPRE 荧光蛋白 CAG EGFP Cre依赖表达 pAAV-CAG-EGFP-3xFLAG-WPRE 荧光蛋白 CAG EGFP 直接表达 pAAV-CAG-mCherry-3xFLAG-WPRE 荧光蛋白 CAG mCherry 直接表达 pAAV-CAG-EGFP-P2A-3xFLAG-WPRE 荧光蛋白 CAG EGFP 直接表达 pAAV-CAG-mCherry-P2A-3xFLAG-WPRE 荧光蛋白 CAG mCherry ...
1. ConVERGD系统的开发与验证:研究团队首先成功开发了ConVERGD系统,该系统融合了自切割核糖核酸酶和FLEx开关,能够在重组酶(如Cre和Flp)的作用下表达特定的分子载荷。通过体外细胞实验,验证了ConVERGD系统在不同条件下表达的特异性。 2. ConVERGD系统的扩展:为了增强系统的多特征交叉靶向能力,研究团队对ConVERGD系统进...