在该研究中,实验人员构建了AAV-EF1α-DIO-YPet-2a-mGFP-WPRE(BrainVTA包装)病毒,并通过将其注射到Tac1-Cre小鼠的l/vlPAG脑区中,实现了对Tac1神经元完整形态的特异性标记(图1)。 该病毒运用EF1α启动子和Cre依赖模式,串联表达了YPet (yellow fluorescent protein for energy transfer)[2]和mGFP (lipid-modi...
PPHP.eB:CAG-DIO-GFP and AAV9:CAG-mNeonGreen was directly labeled with Axexa555-NHS ester. In brief, 1 mM Alexa555-NHS (2 μL in DMSO, 2 nmol) was added to PHP.eB (7 × 1012 vg, 12 pmol) or AA9 (5.2 × 1012 vg, 7 pmol) in PBS buffer (pH ...
microorganisms Article Adeno-Associated Virus (AAV)-Delivered Exosomal TAT and BiTE Molecule CD4-αCD3 Facilitate the Elimination of CD4 T Cells Harboring Latent HIV-1 Xiaoli Tang 1, Huafei Lu 1, Patrick M. Tarwater 2, David L. Silverberg 3 , Christoph Schorl 4,5 and Bharat Ramratnam 1,6...