AAV9-EF1α-DIO-hM3D(Gq)-mCherry1.85×10E13 VG/mLPVN（下丘脑室旁核）：120 nLCeA（中央杏仁...
Gq偶联的DREADD hM3Dq是一个修饰的人M3毒蕈碱受体，对天然配体乙酰胆碱亲和力低，但对合成配体氯氮平N氧...
AAV-hSyn-DIO-hM3Dq-eGFP、AAV-CMV-flex-shVGAT-Egfp、AAV-CMV-flex-shVGLUT-eGFP 文献: Liang HY, Chen ZJ, Xiao H, et al. nNOS-expressing neurons in the vmPFC transform pPVT-derived chronic pain signals into anxiety behaviors. Nat C...
而Gi偶联的hM4Di DREADD，常用于沉默靶向神经元，在CNO刺激下，导致神经元抑制。在DREADDs研究中，有两类主要策略：一是利用病毒注射，通过重组AAV载体，配合细胞特异性启动子和立体定位注射，实现DREADD基因在不同组织细胞内的精准表达。二是采用转基因动物模型，如利用携带DREADD转基因的DIO载体。这两种策...
mOXT AAV-DR391 ssAAV.mOXT-hM3Dq-mCherry-WPRE AAV-DR392 ssAAV.mOXT-DIO-HA-hM3D(Gq)-IRES-mCitrine AAV-DR393 ssAAV.mOXT-HA-hM3D(Gq)-IRES-mCitrine AAV-DR394 ssAAV.mOXT-HA-hM3D(Gq)-mCherry AAV-DR396 ssAAV.mOXT-DIO-hM3D(Gq)-mCherry AAV-DR398 ssAAV.mOXT-DIO-rM3D(Gs)-...
AAV9 [GFAP-hM3Dq-mCherry],≥1E+13 GC/mL,100ul ￥2,580 24h DR112-100 AAV9 [GFAP-DIO-HA-hM3D(Gq)-IRES-mCitrine],≥1E+13 GC/mL,100ul ￥2,580 24h DR113-100 AAV9 [GFAP-HA-hM3D(Gq)-IRES-mCitrine],≥1E+13 GC/mL,100ul ...
AAV9 [mOXT-hM3Dq-mCherry-WPRE],≥1E+13 GC/mL,100ul ￥2,580 24h DR392-100 AAV9 [mOXT-DIO-HA-hM3D(Gq)-IRES-mCitrine],≥1E+13 GC/mL,100ul ￥2,580 24h DR393-100 AAV9 [mOXT-HA-hM3D(Gq)-IRES-mCitrine],≥1E+13 GC/mL,100ul ￥2,580 24h DR394-100 AAV...
孤束核Phox2b神经元参与小鼠呼吸调控 发表期刊:Journal of Neuroscience(IF=5.97) 研究领域:孤束核影响呼吸机制研究 合作单位:河北医科大学 吉凯产品:AAV-EF1α-DIO-hM3Dq-mCherry(化学遗传腺相关病毒)
AAV-hSyn-DIO-hM3Dq-eGFP、AAV-CMV-flex-shVGAT-Egfp、AAV-CMV-flex-shVGLUT-eGFP 文献: Liang HY, Chen ZJ, Xiao H, et al. nNOS-expressing neurons in the vmPFC transform pPVT-derived chronic pain signals into anxiety behaviors. Nat Commun. 2020 May 19;11(1):2501 ...
注射参数病毒类型: AAV-hSyn-HA-hM4Di-IRES-mCitrineAAV-CaMKIIα-eGFPAAV-hsyn-DIO-hM3Dq-eGFPAAV-hsyn-DIO-hM4Di-eGFPAAV-hsyn-DIO-mCherryAAV-CaMKIIα-ChR2-eYFPAAV-CMV-flex-shVGAT-eGFPAAV-CMV-flex-shVGLUT-eGFP 注射部位:小鼠前额叶皮质 ...