AAV-CMV-eGFP (Addgene plasmid #193022) was made by removing the β-globin intron upstream of the eGFP sequence from a plasmid that was gifted from Connie Cepko (Addgene plasmid #67634). AAV-PGK-eGFP (Addgene plasmid #193023) was made by removing the β-globin intron preceding eGFP ...
载体名字 功能基因类型 启动子 荧光 表达方式 pAAV-CMV-MCS-IRES2-EGFP-WPRE 荧光蛋白 CMV EGFP 直接表达 pAAV-CMV-EGFP-3xFLAG-WPRE 荧光蛋白 CMV EGFP 直接表达 pAAV-CMV-EGFP-P2A-3xFLAG-WPRE 荧光蛋白 CMV EGFP 直接表达 pAAV-CMV-EGFP-3xFLAG-tWPA 荧光蛋白 CMV EGFP
Fig. 3 Silencing of SHN3 by systemically delivered rAAV9 promotes boneformation. a Diagram of the rAAV9 construct containing a CMV enhancer/ chickenβ-actin promoter (CB), amiR-ctrl or amiR-shn3, an Egfp reporte...
过表达 GV388 CMV bGlobin-MCS-EGFP-3FLAG-WPRE-hGH polyA 1207 EGFP 融合 GV389 CMV bGlobin-MCS-mCherry-3FLAG-WPRE-hGH polyA 1207 mCherry 融合 GV410 CMV bGlobin-MCS-3FLAG-T2A-EGFP 1130 EGFP 非融合 GV411 CMV-betaGlobin-MCS-3Flag-SV40 PolyA 2819 无 无荧光 GV461 CMV-betaGlobin-MCS-SV4...