4. 全文基于原文:Advanced Analytical Approaches For AAV EmptyFull Capsid Characterization And Quantification (bioprocessonline.com) 5. USP activities on AAV: Standards and Characterization of AAV full-to-empty particles
AAV purity analysis SDS-PAGE, Coomassie Blue Staining, Capsid band size meet reference SDS-PAGE, Silver Staining, Capsid band size meet reference, Capsid band size meet reference - Endotoxin Test LAL, <10EU/mL LAL, <1EU/ml (endotoxin removal may reach <0.2EU/ml) - Mycoplasma - qPCR, Neg...
AAV'sTransduction Efficiencydepends capsid-receptor interaction, cellular uptake and translation. 3 key factors affect AAV transduction success: correctserotypeto tissue match,high titerof capsids and highpercentageof effective capsids. Why use AAV?
Serotype is classified by the type of capsid structural proteins and determines tissue specificity. Serotype 2 (AAV2) is the most well-studied serotype. AAV2 has a broad range of tissue infectivity and is the most popular serotype for research purposes. Serotype 5 (AAV5) and serotype 6 (AA...
Size * Price SL116193 AAV Empty Capsid (Serotype 1) >1x10^13 Capsid/mL 30 µL $168 ~ $488 SL100802 AAV-Null (Serotype 1) >1x10^13 VG/mL 30 µL $168 ~ $488 SL100803 AAV-GFP (Serotype 1) >1x10^13 VG/mL 30 µL $168 ~ $488 ...
Not for use in diagnostic procedures Unit Size (µl) 100 100 Working range (dilution) 1:5000 – 1:20000 1:5000 – 1:20000 No. of ELISA plates (96 wells) 50 – 200 50 – 200 AAV Total Capsid ELISA - recommended starting protocol 1 Coat plate with AAV c...
(4) QUALITY CONTROL OF AAV CAPSID LOADING:PROTAGEN 作者:ZTL-LJ 关键词Keywords:CDE USP 基因治疗药物 杂质分析 空心率 光散射 更多内容 1. RNA药物递送载体表征与分析方法 2. 尺寸排阻色谱与多角度光散射联用对AAV进行全面表征和定量 3. 高通...
Select the AAV CQAs you would like to measure: AAV titer Protein expression potency Empty/full ratio AAV identity Capsid protein ratio AAV purity AAV stability BiodistributionWhich sample type are you using?Crude samples (e.g. cell/tissue lysates)...
(AAV) capsids after intravenous delivery, which we achieved by employing a Cre-transgenic-based screening platform and sequential engineering of AAV-PHP.eB between the surface-exposed AA452 and AA460 of VP3. From this selection, we identified capsid variants that were enriched in the brain and...
建议稀释比 已发表文章 免疫印迹 (WB) 1:500-1:1,000 - 产品规格 种属反应 Virus 宿主/亚型 Rabbit / IgG 分类 Polyclonal 类型 Antibody 抗原 Directed against a synthetic peptide within residues 450-475 of 81 kDa capsid VP3 protein of AAV 4. ...