Our standard Capsid Engineering service include capsid library design and construction, two rounds of screening and final validation of top variant performance in model animals. Please discuss with our technical support for your screening criteria, animal test and any customized needs. Detailed workflow ...
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Capsid Development Naturally occurring serotypes have long enabled delivery of AAV gene therapeutics. However, precise tissue targeting without off-target implications important for advancing therapeutic performance. Rational design for chimeric capsids with a range of possibilities A critical component of ...
Preserve ITR INTEGRITY in aav vectors Truncated ITRs can reduce the yield of full viral capsid production and increase generation of undesirable non-rAAV encapsidated DNA. Unfortunately, AAV plasmid ITRs are frequently mutated during plasmid production in bacterial cells. GENEWIZ AAV plasmid preparation...
AAV capsid engineering, especially direct evolution engineering, can generate highly specific serotypes that target muscle and/or heart [3]. However, new capsid engineering is a laborious process of screening millions of capsid variants before the desired specificity is met [3]. This is a multi-...
In this application note, we show how imaged capillary isoelectric focusing (icIEF) technology on the Maurice system can be used to characterize empty, intermediate and full AAV capsids at native and stability screening conditions, providing robust and reproducible data. With this, the Maurice system...
(AAV) capsids after intravenous delivery, which we achieved by employing a Cre-transgenic-based screening platform and sequential engineering of AAV-PHP.eB between the surface-exposed AA452 and AA460 of VP3. From this selection, we identified capsid variants that were enriched in the brain and...
AAV Capsid Evolution Enhancer/Promoter Screening AAV Biodistribution Profiling Recombinant Protein ExpressionNew Bacterial Protein Expression Mammalian Protein Expression Insect Protein Expression Cell-Free Protein Expression BAC Recombineering Mutagenesis CDMO Services ...
AAV vectors for capsid screening were produced in small scale by triple-transfecting HEK293T cells using polyethylenimine (PEI) with (i) an AAV vector plasmid, (ii) an AAV helper plasmid expressingrepandcap, and (iii) an adenoviral helper plasmid72. The capsids used were AAV-DJ44, AAV-DJ...
Recombinant AAV capsid proteinProvided herein are methods of selective screening. In addition, various targeting proteins and sequences, as well as methods of their use, are also provided.Benjamin E. DevermanPaul H. PattersonViviana Gradinaru