Students are requested to go through the Syllabus first of all the things before even starting their preparation. Make notes of everything that you study as it is easier to refer to notes than going through all over again. Daily revision is an important aspect as what you’ve studied becomes...
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PREPARATION OF WORKING SOLUTION Phalloidin-iFluor™ 680 Conjugate working solution Add 1 µL of Phalloidin-iFluor™ 680 Conjugate solution to 1 mL of PBS with 1% BSA. NoteThe stock solution of phalloidin conjugate should be aliquoted and stored at -20 °C. protected from light. ...
PREPARATION OF STOCK SOLUTIONS Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles Protein stock solution (Solution A) Mix 100 µL of a reaction buffer (e.g., 1 M sodium ca...
Working on audit and accounts preparation assignments with your team, Working closely with your mentor on audit planning memorandum, Analytical reviews, Drafting client letters, Preparing accounts and producing sets of financial statements. Studying towards Professional Qualification (AAT Level 3, ...
2.2用PBS冲洗固定的细胞2-3次。 2.3可选:在PBS中加入0.1%Triton X-100固定细胞(步骤2.2)3至5分钟,以增加渗透性。 用PBS冲洗细胞2-3次。 2.4将100μL/孔(96孔板)的iFluor 488-鬼笔环肽工作溶液(来自步骤1)加入固定的细胞(来自步骤2.2或2.3),并在室温下将细胞染色15至60分钟。 2.5用PBS轻轻冲洗细胞2至3次...
Tracing of axonal connectivities in a combined slice preparation of rat brains--a study by rhodamine-dextran-amine-application in the lateral nucleus of the amygdala Authors: von Bohlen und Halbach, O., Albrecht, D. Journal: J Neurosci Methods (1998): 169-75 ...
Level 3: Advanced Bookkeeping and Financial Accounts Preparation have been combined into one unit called Financial Accounting: Preparing Financial Statements (FAPS) Level 4: Budgeting and Decision & Control have been combined into Advanced Management Accounting (AMAC) Additional changes The End Point ass...
Monitor the fluorescence increase at Ex/Em= 330/520 nm (Cutoff = 475 nm), fluorescence microscope with DAPI filter set Important notes Thaw all the components at room temperature before starting the experiment. PREPARATION OF WORKING SOLUTION ...