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Free-floating sections containing the LC/SN region were washed in 0.1 M PB and quenched with 3% H2O2and 10% methanol for 15 min. After a second wash, sections were blocked in 5% normal donkey serum with 0.3% Triton X-100 in 0.1 M PB for 1 h before incubating them overnight with prim...
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