摘要: 目的 探讨BRAFV599E突变对黑素瘤生长的作用。方法 使用BRAF表达受到抑制的黑素瘤细胞Abraf1和Abraf2,以及BRAF表达未受抑制的A375细胞和Aneg细胞,MTT比色实验和平板克隆形成实验分别检测4种细胞的生长和克隆形成能力,流式细胞仪检测细胞周期和凋亡的变化。结果 与A375和Aneg细胞相比,Abraf1和Abraf2细胞的体外...
angulata had an inhibitory effect on the v-raf murine sarcoma viral oncogene homolog B1 (BRAF)-mutated melanoma A375 cell line at the concentration of 20 mg/ml, and with the bioactivity-guided fractionation approach, physalin B has been determined to be the main active compound (data not ...
The present study investigated the correlation between CD147 expression and Raf-MEK-ERK signaling in MM using the A375 human MM cell line, which harbors the activating mutation of BRAF. The phosphorylation of epidermal growth factor receptor (EGFR) was upregulated, and mitogen-activated protein ...
Oncogenic BRAF regulates oxidative metabolism via PGC1α and MITF. Cancer Cell 2013, 23, 302–315. [Google Scholar] [CrossRef] [PubMed] Ferguson, J.; Smith, M.; Zudaire, I.; Wellbrock, C.; Arozarena, I. Glucose availability controls ATF4-mediated MITF suppression to drive melanoma cell ...
In patients with BRAF-mutated melanoma, MEK inhibitors are more frequently used in association with BRAF inhibitors to definitely block ERK activity and the associated cell proliferation [52]. Unfortunately, these treatments do not have long-lasting effectiveness because drug resistance occurs soon [53...